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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 474 results for: mag

Showing: 41 - 60 of 474 results

  1. Open Enzyme Collection

    Type
    Collection
    ...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

  3. Viral Production

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    Collection
    ...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with ...

  5. COVID-19 Resources

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    Collection
    ...SARS-CoV-2 is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics...

  6. Rett Syndrome

    Type
    Collection
    ... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

  9. Plan Your Experiment

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    Collection
    ...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...

  10. Biosensor AAV Preps

    Type
    Collection
    ...none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Boyden...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

  12. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...open the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split...wash again, then image. But here’s where the “sequential” part comes in. After you image the first target....1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...ve captured your images, various software programs will allow you to align the images of each labeled ...maybe you just want to make some really beautiful images? Multiplex IF may be just what you need! How does...of primary and secondary antibody labeling and imaging, repeating for each target. Alternatively, you ...Sequential Multiplex IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins...proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM, target proteins (expressed...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...articles. Here's what you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination...Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher...and minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds ...

  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...order to reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following...Fluorescent Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging Catch Up on Your GFP...would otherwise be missed with conventional FP imaging. PA-FPs are divided into 2 main categories - those...photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation Irreversible...phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another ...fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2: Properties of Selected...

  16. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...Individual images as well as collections of images are easy to download in a .png file format. Images are under...indicate if changes were made to the images. This requirement means the images are probably better suited for...than for journal publications. Google Image Search Google image search is a great tool for searching the...can search for images “Labeled for reuse with modification.” Occasionally Google Image Search results ...results includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...your public library. If you want to use pre-drawn images in your figures Biorender Biorender is like clip...create it. Additionally, you can upload your own images to the Biorender workspace. Free Biorender accounts...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was magnetically...revealed that Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids...is multiplex automated genome engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate...use the MAGE method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses.... Methods 2016. PubMed PMID: 27798611 Magneto 2.0 - A magnetic remote control for the nervous system ... 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway...Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4...

  18. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...excitation microscopy is a fluorescence imaging technique that allows imaging of living tissue up to about one... technique and the in vivo live-cell imaging (intravital imaging) studies it’s enabled are revealing the...immune cell mobility than the static microscopy images of chemically coloured lymphoid organs did in the...Two-photon microscopy was the next revolution in cell imaging, giving researchers the ability to monitor cellular...than any other cell type in the body! Intravital imaging powered by two-photon microscopy has uncovered ...infected tissues. Before the use of two-photon imaging, our understanding of pathogen-immune cell interaction...Neutrophils travel to the sites of infection or damage (Peters et al. 2008, Chtanova et al. 2008, Kamenyeva...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluorescent protein fusions for imaging Fluorescent proteins are fantastic imaging tools but their use is limited...for imaging. These authors set out to design a better fluorescent protein that could be co-imaged with...of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak is at 460 nm thus...thus making it non-optimal for in vivo imaging. The solution is to couple Nluc with a red-shifted fluor....LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused to enhanced ... The isolated cells were injected into mice and imaged after i.p. or i.v. administration of the furimazine...of why a BRET reporter can improve deep tissue imaging. The constructs for GpNLuc and OgNLuc are available...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed...milliseconds to acquire one image, thereby significantly reducing the time it takes to image through a large z-...the acquired image (5).  A way to get around this problem is to illuminate and acquire images of a sample...the 3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal... axial resolution and reduce phototoxicity for imaging live specimens, they combined light sheet microscopy...sequential multiple-view reconstruction (5). In order to image large tissue such as cleared rodent brain, the Ultramicroscope...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens that ...
Showing: 41 - 60 of 474 results