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We narrowed to 477 results for: mag

Showing: 41 - 60 of 477 results

  2. TALEN Guide

    Type
    Collection
    ...December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region of DNA...

  3. Open Enzyme Collection

    Type
    Collection
    ...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

  5. Viral Production

    Type
    Collection
    ...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with ...

  6. COVID-19 Resources

    Type
    Collection
    ... Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics...

  8. Rett Syndrome

    Type
    Collection
    ... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

  11. Plan Your Experiment

    Type
    Collection
    ...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...

  12. Biosensor AAV Preps

    Type
    Collection
    ...none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Boyden...

  13. Chemogenetics Guide

    Type
    Guide
    ...activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition Magnus et al.,...activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition Magnus et al.... 792, uPSEM 817 Cl - influx Neuronal inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792,... PSEM 89S Na + , K + influx Neuronal activation Magnus et al., 2011 LMOs Luminopsins (LMOs), or luminescent...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3: Descriptions....org/10.1016/j.isci.2025.112022. PMID: 40092615 Magnus, C. J., Lee, P. H., Atasoy, D., Su, H. H., Looger.../doi.org/10.1126/science.1206606 PMID: 21885782 Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

  14. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

  15. CRISPR Guide

    Type
    Guide
    ...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

  17. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...

  18. Modular Cloning Guide

    Type
    Guide
    ...Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. MoClo Plasmid Kits Available...Acinetobacter spp. and Acinetobacter baumannii . Komagataeibacter Tool Kit (KTK) Bacterial Expression Tom Ellis...constructs expressed in the cellulose-producing Komagataeibacter species. Ralstonia-GG-Kit Bacterial Expression...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...open the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split...wash again, then image. But here’s where the “sequential” part comes in. After you image the first target....1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...ve captured your images, various software programs will allow you to align the images of each labeled ...maybe you just want to make some really beautiful images? Multiplex IF may be just what you need! How does...of primary and secondary antibody labeling and imaging, repeating for each target. Alternatively, you ...Sequential Multiplex IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins...proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM, target proteins (expressed...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...articles. Here's what you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination...Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher...and minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds ...
Showing: 41 - 60 of 477 results