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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 470 results for: mag

Showing: 41 - 60 of 470 results

  1. Open Enzyme Collection

    Type
    Collection
    ...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

  3. Viral Production

    Type
    Collection
    ...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with ...

  5. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics...

  6. Rett Syndrome

    Type
    Collection
    ... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

  8. Bacterial Expression Systems

    Type
    Collection
    ...!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...

  10. Biosensor AAV Preps

    Type
    Collection
    ...none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Boyden...

  11. Chemogenetics Guide

    Type
    Guide
    ...activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition Magnus et al.,...activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition Magnus et al.... 792, uPSEM 817 Cl - influx Neuronal inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792,... PSEM 89S Na + , K + influx Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions...Huntington's Disease Pathogenesis. iScience , in press. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson...96. PMID: 21885782 (Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

  12. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

  13. CRISPR Guide

    Type
    Guide
    ...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

  14. Cloning

    Type
    Guide
    ...gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to ...Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning...gateway cloning. Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based...chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top ...your next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to ...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

  16. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...open the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split...wash again, then image. But here’s where the “sequential” part comes in. After you image the first target....1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...ve captured your images, various software programs will allow you to align the images of each labeled ...maybe you just want to make some really beautiful images? Multiplex IF may be just what you need! How does...of primary and secondary antibody labeling and imaging, repeating for each target. Alternatively, you ...Sequential Multiplex IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins...proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM, target proteins (expressed...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...articles. Here's what you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination...Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher...and minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds ...

  19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...order to reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following...Fluorescent Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging Catch Up on Your GFP...would otherwise be missed with conventional FP imaging. PA-FPs are divided into 2 main categories - those...photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation Irreversible...phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another ...fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2: Properties of Selected...

  20. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...Individual images as well as collections of images are easy to download in a .png file format. Images are under...indicate if changes were made to the images. This requirement means the images are probably better suited for...than for journal publications. Google Image Search Google image search is a great tool for searching the...can search for images “Labeled for reuse with modification.” Occasionally Google Image Search results ...results includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...your public library. If you want to use pre-drawn images in your figures Biorender Biorender is like clip...create it. Additionally, you can upload your own images to the Biorender workspace. Free Biorender accounts...
Showing: 41 - 60 of 470 results