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...somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments. The C....

...Link opens in a new window) , including: data and images from the hiPS cell lines gRNA sequences used in...

...December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region of DNA...

...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in...

...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with ...

...genome editing of the moss P. patens . CRISPR Magdalena Bezanilla 1000000270 ENABLE® Gene Editing in planta...

... Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics...

...SigmaR1 constructs . (Sawyer et al. Dev Cell. 2024) Image exocytosis with VAMP2/3 plasmids to study CNS myelination...

... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

...brightness, assessed using ssRNAseq or high-resolution imaging. For more details, visit the Allen Institute's ...

...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...

...activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal inhibition Magnus et al.,...activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal inhibition Magnus et al.... 792, uPSEM 817 Cl - influx Neuronal inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792,... PSEM 89S Na + , K + influx Neuronal activation Magnus et al., 2011 LMOs Luminopsins (LMOs), or luminescent...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3: Descriptions....org/10.1016/j.isci.2025.112022. PMID: 40092615 Magnus, C. J., Lee, P. H., Atasoy, D., Su, H. H., Looger.../doi.org/10.1126/science.1206606 PMID: 21885782 Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

...Flp dependent 8 Karl Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Edward...

...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

...Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. MoClo Plasmid Kits Available...Acinetobacter spp. and Acinetobacter baumannii . Komagataeibacter Tool Kit (KTK) Bacterial Expression Tom Ellis...constructs expressed in the cellulose-producing Komagataeibacter species. Ralstonia-GG-Kit Bacterial Expression...

...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...