We narrowed to 61 results for: zebrafish

Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast and FLASH

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish, plants, and human cells

CRISPR plasmids for precisely editing DNA bases without inducing a DNA break.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab

CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.

Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Guide to TALEN technology and using TALEN plasmids

List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

CRISPR plasmids for endogenous tagging of your gene of interest.

The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience research. Find plasmids encoding these R-mAbs here.

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.