We narrowed to 84 results for: zebrafish
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TypeBlog Post...in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation...
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Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection...for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The...CRISPR-Cas/RGN expression plasmids for use in zebrafish Guide RNA expression plasmids for the EGFP Reporter... Guide RNA expression plasmids for endogenous zebrafish genes... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...TALEN plasmids targeting zebrafish genes.... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous...Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce...induce targeted mutations with high efficiency in zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang ...MGH to target sites within various endogenous zebrafish genes; many of these were generated as part of...effort to provide gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus. Mammalian ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker... -
Cre-lox system
TypeCollection...61391 pCS2-Cre.zf1 Cre codon modified for zebrafish CMV Zebrafish Burgess 61570 Pvalb-2A-Cre targeting vector...Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10 Zebrafish Stainier 85040...for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional Resources Cre-dependent ... ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre...Lentiviral Jacks 60877 pMAZe Cre and dtomato hsp70 Zebrafish Lewis 60930 pPL5071_TEF1*-Cre_URA3 Cre expressed...pME-ERT2-Cre-ERT2 Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK...fli1aCreERT2 Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 Cre; NeoR act15 ... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...pCS2+8CCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans...Expression pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/C.elegans/Sea...pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus/C.elegans... pCS2+8CmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/C.elegans/... pCS2+8CmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/...of GFP and/or mCherry, with neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans Hamdoun Lab... -
CRISPR Plasmids and Resources
TypeCollection...designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in zebrafish....mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR... -
CRISPR Plasmids - Base Edit
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker... -
TALEN Engineering
TypeCollection... in: C. elegans ( Wood et al., Science 2011 ) Zebrafish ( Sander et al., Nat Biotechnol. 2011 ) Rats (...REAL, REAL-Fast and FLASH TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection... pT7-gRNA 46759 Zebrafish BsmBI none S. pyogenes Chen and Wente DR274 42250 Zebrafish BsaI none S. pyogenes...U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish BseRI none S. ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... Lu pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre...Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 74010 Zebrafish U6 yes, cut S. pyogenes Cre Del Bene pTE4396 74041 Mammalian...Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using ...based pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...has been used to engineer ZFNs that function in zebrafish, plants, and human cells...has been used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol ... -
CRISPR References and Information
TypeCollection...cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish, mouse, rat, and human. Developed by the Stanley...Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis...Arabidopsis, and rice. Proof-of-concept performed in zebrafish. Developed by the Jason Berman Lab . CRISPRdirect...orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...Resources collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources...fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector with multiple cloning ...destination vector for mammalian, avian, xenopus, or zebrafish expression GST plasmids made by Addgene MBP Protein... -
Zinc Finger Consortium Reagents
TypeCollection...Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... -
CRISPR Plasmids - Epigenetics
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... -
CRISPR Plasmids - RNA Editing
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...