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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 291 - 300 of 677 results

  1. Science Career Options

    Type
    Blog Post
    ...to graduate from university, many students are confronted with the question ‘what now?’ This is often a...good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...exercise these skills outside of a formal work environment also shows that you are passionate and capable...

  2. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...to create a more productive and cohesive team environment. Neglecting communication skillsClear communication... great "baby project," offering a supportive environment to build my management skills. Finally, developing...effectively and foster a cohesive, productive environment. For example, in my current role managing a cross-functional...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ..., which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the protein...of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary. This....com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...us, as much as it as about the amazing years in front of us. So in our last celebratory moment, we wanted...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing...associated with gRNA introduction (Allen, et al., Frontiers in Genome Editing). This is particularly important...CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... these conditions. On top of these factors, environmental conditions such as cellular redox state and ...et al, 2012). The third issue relates to the environment of the fluorescent protein. Photostability measurements...in vitro methods allow for a well-controlled environment, but they do not mimic the natural situation....

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ... of a toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during...cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin protein (CcdA) that protects... 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353...
Showing: 291 - 300 of 677 results