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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 648 results for: ato

Showing: 601 - 620 of 648 results

  3. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible...tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

  4. Deep Dive: qPCR

    Type
    Blog Post
    ...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang, F. (2013). Double nicking...

  6. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes...conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead... mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip ...swirl 5–10 times to mix. Return the flask to the incubator. Section 3: BCD Feed and valproic acid supplementation...

  8. Centrifugation

    Type
    Protocol
    ... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...Introduction A centrifuge is a common piece of laboratory equipment used to separate different components...

  9. Immunocytochemistry

    Type
    Protocol
    ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

  10. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...difficult to find. Leave the cells undisturbed in an incubator for 7–14 days. After ~7 days, scan the plate for...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents...time for a total of eight electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm ...

  13. Protocol - How to Streak a Plate

    Type
    Protocol
    ...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...

  14. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may... temperature. g. Retrieve HEK-293T cells from incubator. The cells should be 50-80% confluent and in DMEM...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...

  16. Water Bath Protocol

    Type
    Protocol
    ... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...

  17. Video Library

    Type
    Protocol
    ... Protocols Video protocol guides for standard laboratory procedures. (Link opens in a new window) Video...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...

  19. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    .... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

  20. Colony Formation Titering Assay

    Type
    Protocol
    ...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...Swirl gently to mix and place the dish in the incubator. Incubate for 48–72 h. Pro-Tip Different cells...
Showing: 601 - 620 of 648 results