Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 678 results for: RON

Showing: 311 - 320 of 678 results

  1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ... 0 and 100 for each guide, with 100 being the strongest interaction between the guide and the target and...interactions (e.g., scores <20) are reduced to 0 and strong interactions (e.g., scores >80) are transformed...weak gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much as perfect...

  2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT...

  3. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...possible because baculoviruses are slightly less prone to recombination than other viruses, like lentivirus.... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https:...

  4. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...Compensation beads are a convenient tool to obtain a strong positive signal for a given fluorophore, as they...won’t bind anything (negative population). It is strongly recommended that when you first run a new panel...compensation beads are convenient in that they provide a strong positive signal, even for rare cellular markers...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...genomic site can be edited based on its chromatin environment. After selecting the best pegRNA design with ...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, C., Choi, J., Chardon, F. M., McDiarmid...

  6. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there...technology: because they necessarily alter the shared environment, they must never be used without popular support2...organisms and associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...adenovirus-based gene therapy clinical trial gone horribly wrong. It brought the whole field to a grinding stop and...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...Applications of CRISPR-Based Genome Editing in the Retina. Front Cell Dev Biol. 2018 May 14;6:53. Review. PubMed ...

  8. MXS Chaining

    Type
    Blog Post
    ...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...resulting HeLa cells show robust labeling, with strong expression and detection of each fluorophore at...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...cost-effective, timesaving for transformations. Cons: Prone to satellite colony formation on agar plates, less...less stable at high temperatures and in acidic environments.   Carbenicillin   Carbenicillin, colloquially...antibiotic than amp: it is more stable in high pH environments and is less susceptible to degradation in agar...
Showing: 311 - 320 of 678 results