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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 621 - 640 of 706 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

  2. Antibody Production

    Type
    Collection
    ...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

  3. Retrovirus Plasmids

    Type
    Collection
    ...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

  4. Promega Plasmid Collection

    Type
    Collection
    ... in a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection... form an active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT... detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT...

  5. TALEN Guide

    Type
    Collection
    ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

  6. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...may also be fused to gRNA sequences to produce fluorescence at a target region, as in the CRISPRainbow kit...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Mammalian U6x2 yes, nick S. pyogenes Puro Jackson pBluescriptSKII+ U6-sgRNA(F+E) empty 74707 Mammalian hU6 none...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit ...simultaneously repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions for other...

  8. Lentivirus Plasmids

    Type
    Collection
    ... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector...Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

  10. Optogenetics AAV Preps

    Type
    Collection
    ...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein ...

  11. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...

  13. Plan Your Experiment

    Type
    Collection
    ...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

  14. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...biological context. Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

  16. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

  17. Genetic Code Expansion

    Type
    Collection
    ...Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao Liu 164195 pCMV-MtAcKRS...Liu 164196 pCMV-MfBulKRS (human-opti) BulKRS M. flavescens acetyl-lysine Mammalian Tao Liu 164579 pEVOL-...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

  19. Synthetic Biology - Overview

    Type
    Collection
    ...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...
Showing: 621 - 640 of 706 results