...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein ...

... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific... Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins:...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...plasmids for your next experiment. For a full description of different domains used in these plasmids, ...Glossary of Optical Switches Category Variant Description Peak Response Spectra (nm) BLUF domains bPAC ...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

...Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao Liu 164195 pCMV-MtAcKRS...Liu 164196 pCMV-MfBulKRS (human-opti) BulKRS M. flavescens acetyl-lysine Mammalian Tao Liu 164579 pEVOL-...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...

...cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated...Engineering newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression...

...biological context. Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

...10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...are based on the pTT3 plasmid backbone which is described by Durocher et al. Nucleic Acids Research 2002...showing how AVEXIS works with a detailed protocol description: Avidity based Extracellular Interaction Screening...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

...envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...

...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

...plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV...Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

...lentivirus technologies. ID Plasmid Generation Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev;...table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...

..., reverse primer pBluescriptKS TCGAGGTCGACGGTATC For pBluescript vector pBluescriptSK TCTAGAACTAGTGGATC...TCTAGAACTAGTGGATC For pBluescript vector pBMN 5' GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector...