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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 678 results for: RON

Showing: 321 - 330 of 678 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    ...antibiotic resistant cells could repopulate the environment after treatment. The phagemid systems used in...pathogenic or drug-resistant bacteria. It’s a bit ironic that CRISPR, the bacterial immune system, may someday...4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron. “Temperate and lytic bacteriophages programmed...

  2. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance.... spouse won’t be at every school performance, chaperone every field trip or cheer at every soccer game...birth and breastfeed. Keeping this always at the forefront will go a long way toward alleviating the guilt...

  4. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...analysis window shows a green checked circle in front of the sample name. If there was a minor error during... error, you will see a red exclamation point in front of that sample. You can hover over the yellow or...line represents the cut site. Cutting and error-prone repair typically result in mixed sequencing bases...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... can be from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such ... A potential solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice...Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

  7. Five Popular Model Organisms

    Type
    Blog Post
    ...research for over a century but was brought to the forefront by Thomas Hunt Morgan, who is known as the “father...work with as they can survive in a variety of environmental conditions, and double every 2 hours. Yeast ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy... and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...and Ruddock., 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing ...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...and screened with in vitro assays to select for strong binders. Then the sequences for the variable domains...
Showing: 321 - 330 of 678 results