We narrowed to 972 results for: Abo;

1. Hot Plasmids: Summer 2024

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   ...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...

3. Viral Vectors 101: Optogenetic Tools

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   ...which tools to use, and what do you need to know about them to be successful in your experiments? In this...

4. Viral Vectors 101: AAV Variables That Matter

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   ...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

5. Antibodies 101: Multiplex Immunofluorescence

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   ...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

6. Technique: Probe Phage Genomes for Host Binding Proteins

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   ...our planet, but we still know collectively little about how they infect and influence the evolution of their...

7. How to Design Your gRNA for CRISPR Genome Editing

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   ...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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   ... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

9. How to Deposit Your Plasmids with Addgene

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   ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

10. Deep Dive: qPCR

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    ... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

11. Technical Design of a Western Blot

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    ...If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices...

12. Using AAV for Neuronal Tracing

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    ...usually severely impaired, and only few reports exist about dye molecules that are transported from one neuron...