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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 678 results for: RON

Showing: 331 - 340 of 678 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should be widely applicable...marking cell types in the nervous system By Ashley Waldron The mammalian nervous system is composed of myriad...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...plasma HIV-1, they are at increased risk for other chronic diseases, including dementia, gut disorders, nerve...disease risk is attributed to latent HIV reservoirs, chronic inflammation, and negative metabolic effects of...in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed... fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance...not degraded, cell cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...
Showing: 331 - 340 of 678 results