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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 730 results for: ACE

Showing: 681 - 700 of 730 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...included between the ITRs is limited by the physical space available inside of the small AAV capsid and directly...information to a given region. This allows researchers to trace connections between neurons, map the brain, and ...Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR... viral vectors for enhanced gene transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/...by stimulating elongation and stabilizing the displaced single-stranded DNA. VA in trans Adenoviral gene...Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle. Immunogenicity...

  2. Optogenetics Guide

    Type
    Guide
    ...better tissue penetrance, which may allow you to place the optic fiber outside of the brain, rendering ... activated by the light is controlled via the placement of the optic fiber. Using viral vectors for opsin... the toxin's local concentration near the cell surface, and enabling the ion channel to function. TULIPs...

  3. Molecular Biology Reference

    Type
    Guide
    ...mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock...Adenine, Cytosine, Guanine and Uracil (U). Uracil replaces thymine in RNA molecules. Every 3 nucleotides ...

  4. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems,...

  6. Promoters

    Type
    Guide
    ...Promoters are about 100-1000 base pairs long and are adjacent and typically upstream (5’) of the sense or coding...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, this region is removed in 5’ LTRs and replaced with a heterologous promoter (usually CMV or RSV...

  8. Plan Your Experiment

    Type
    Guide
    ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids, this region is often removed in 5’ LTRs and replaced with a heterologous promoter (CMV or RSV). 5' ...

  10. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...the trace file, you will see that these base calls are unreliable. This is an example of a trace file...with the sequence, trace file, and primer used. What program can I use to view my trace file? There are ...You should receive your sequencing results as a trace file (.ab1) which graphically depicts the sequence...four nucleotide bases. This is an example of a trace file from a high-quality portion of a sequencing...and include: The sequencing result The original trace file (.ab1 file) provided by your sequencing company...sequencing result, what should I do? Check your trace file first; the apparent mismatch/mutation may be... be the result of a mis-called peak in the trace file. If the mutation is not an artifact, please email...

  11. Water Bath Protocol

    Type
    Protocol
    ...bath when they are placed inside. Water bath floats can be used to secure tubes in place and water bath weights... hold bottles in place. After putting your tubes or bottles in the water bath, place the lid back on. ...bottles and containers. Water baths can also be placed in walk-in refrigerators to achieve a temperature...water, to prevent salts from accumulating on the surfaces of the water bath as the water evaporates from...bottle, for example, number of drops per liter. Place a thermometer in the water bath. Oftentimes, the...bath will have a spot to secure the thermometer in place. Turn the water bath on and set the appropriate ... Water baths tend to heat up relatively slowly. Place the water bath cover on the top of the water bath...

  12. AAV ddPCR Titration

    Type
    Protocol
    ...mini centrifuge and place on ice. Wipe down a DG8 cartridge holder with bleach and place in the Biological...Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer block and place in the dilution...temperature is reached, place the PCR plate with the foil onto the metal support block. Place the block in the... on ice before use. Wipe down all pipettes and surfaces with 10% bleach. Safety Warnings AAV is generally... BSC until ready to use. Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block...spin in a mini centrifuge for 10 sec before use. Place an 8-well PCR tube strip into a chilled 96-well ...1X dilution buffer from step 9 to the NTC tube. Place a DG8 cartridge into the cartridge holder. Using...

  13. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...running buffer and 5 μL of EtBr, place on a rocker for 20-30 mins, replace EtBr solution with water and destain...the agarose into a gel tray with the well comb in place. Pro-Tip Pour slowly to avoid bubbles which will... the sides/edges of the gel with a pipette tip. Place newly poured gel at 4 °C for 10-15 mins OR let sit...in a hurry, the gel will set more quickly if you place the gel tray at 4 °C earlier so that it is already...instead of diffusing in the buffer. Once solidified, place the agarose gel into the gel box (electrophoresis... you had not added EtBr to the gel in the first place. Carefully load a molecular weight ladder into the...prevent bubbles or buffer from entering the tip. Place the very top of the tip of the pipette into the ...

  14. Centrifugation

    Type
    Protocol
    ...pellets form in approximately the same place in each sample. Place the centrifuge’s internal lid on the ... appropriate for the biosafety level of the lab space Reagents Sample to be centrifuged Water Background...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...the lid open, the rotor with microfuge tubes in place, and the control panel where you can set the time...picture above. Wear PPE appropriate for the lab space in which you are working. Even if your samples may...specific PPE, you never know what else has been placed in the centrifuge before you and may still be on...that everything appears to be working smoothly. Place your sample tubes into the rotor so that they are...

  15. Western Blot

    Type
    Protocol
    ... Stack in the plastic tray. Place the Bottom Stack on the blotting surface. Align the electrical contacts...blotting surface of the iBlot 2 Gel Transfer Device. Wet the pre-run gel in deionized water and place it on... Load 5–10 µL of the prestained protein ladder. Place the lid on the tank and plug the electrode cords...piece of iBlot Filter Paper in deionized water. Place the pre-soaked iBlot Filter Paper on the gel and...and remove the air bubbles with the roller. Place the Top Stack over the pre-soaked filter paper. Remove...Remove air bubbles using the roller. Place the Absorbent Pad on top of the Top Stack such that the electrical...corresponding electrical contacts on the blotting surface of the iBlot 2 Gel Transfer Device. Close the lid...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...mini centrifuge and place on ice. Wipe down a DG8 cartridge holder with bleach and place it in the Biological...temperature is reached, place the PCR plate with the foil onto the metal support block. Place the block in the... on ice before use. Wipe down all pipettes and surfaces with 10% bleach. Safety Warnings Lentivirus is...touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block...spin in a mini centrifuge for 10 sec before use. Place an 8-well PCR tube strip into a chilled 96-well ... forth 10 times to mix. Generating the Droplets Place a DG8 cartridge into the cartridge holder. Using...dispensing the droplets down the side of the tube. Place a Pierceable Foil Heat Seal on the PCR plate with...

  17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store...volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to mix. Optional: Place the tube either at...isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized DNA Purification...phase (protein) Pipet the aqueous DNA layer and place it in a new microfuge tube. Add equal volume of ...

  18. Weighing Reagents Protocol

    Type
    Protocol
    ...accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing out an amount larger... the sucrose in your container after each time. Place the weighing boat or weighing paper onto the balance...fold it in half first to create a crease and then place it unfolded onto the balance. This will help you... on as otherwise, the paper could stick to the surface. Tare the weighing boat or paper. You need to do...volume (e.g. 0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance and then tare...Pro-Tip When you weigh out a reagent, you may leave trace amounts of it on the balance. Therefore, it’s best...

  19. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...experiment, make sure your workspace is clean and uncluttered. Disinfect your workspace with appropriate materials...Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps container ...times for each agent. Make sure you have enough space to work at your lab bench. It’s possible that while...or biohazardous materials are splashed onto your face and/or get into your eyes. Wash your hands before...able to close securely. Biohazard signs should be placed in all areas containing biohazardous materials,...

  20. Pipetting Protocol

    Type
    Protocol
    ...represents the first decimal place. For the P20, this represents the second decimal place, and for the P2 this...receiving tube, place the pipette tip into the liquid. If this is a new container, place the tip near the...this represents the third decimal place. Procedure How to Pipette Now that you know the different parts ...indicate that the tip is not on the pipette properly. Place the pipette tip into the container you are using...
Showing: 681 - 700 of 730 results