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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 770 results for: Dos;

Showing: 721 - 740 of 770 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...thicker as the proteins and DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic DNA will ...4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down sink. Top Protocol: Ethanol Precipitation...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use...incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that are over 30 passages. Section ...

  3. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary to template strand). However, primers do not need to correspond to the template strand completely...

  4. Typing CRISPR Systems

    Type
    Blog Post
    ...adaptive immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... a new or experienced reprogrammer? Which methods do you have experience using? Let us know about your...multiple transfection makes it difficult to control the dose of plasmid the cells receive over the whole reprogramming...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation. Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...surface. Tare the weighing boat or paper. You need to do this because you don’t want to include the weight...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...entire dilution plate with Microseal adhesive seal - do not press the film, just gently cover so nothing ...collecting drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the ...

  11. Water Bath Protocol

    Type
    Protocol
    ...of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles or tubes...

  13. Video Library

    Type
    Protocol
    ...Protocol Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise the ion pump’s function or cause cellular...

  15. Centrifugation

    Type
    Protocol
    ... centrifuge and be dangerous for the user. If you do not have a balanced number of samples, you may need...

  17. Immunocytochemistry

    Type
    Protocol
    ... and a negative control sample such as cells that do not express the protein of interest....titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning...

  18. General Transfection

    Type
    Protocol
    ...8x10 5 cells in a T75 flask in a volume of 15 mL. Do not add antibiotics to the media. Use cells that ...

  19. Lentivirus Production

    Type
    Protocol
    ...10 5 cells in a T75 flask in 15 mL DMEM Complete. Do not add pen-strep to the media. Use cells that are...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...designed by Eric Greenwald and the Jin Zhang Lab at UCSD Do you have suggestions for other plasmids that should...Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic GMP) Green fluorescent probe for ...
Showing: 721 - 740 of 770 results