We narrowed to 986 results for: des

1. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...molecular and microbiology for the last two decades as a staff scientist, but before going to graduate ...

2. Changing Labor Laws Bring Increased Postdoc Wages

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   ...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

3. Getting the Most from Your Lentiviral Transduction

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   ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ... live cells in real time. Previously, we have described a number of practical considerations that should... herpesvirus.       References 1. Snapp, Erik. "Design and use of fluorescent fusion proteins in cell ...

5. Synthetic Biology - Networks and Gene Regulation

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   ...related to Networks and Regulation. The collection includes both naturally ocurring and synthetic regulatory...

6. Zinc Finger Consortium Reagents

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   ...and educational use. Links to additional pages describing these reagents are provided below: Zinc Finger...

7. CRISPR Plasmids - dCas9-FokI

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   ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive...

8. iPSC Neurodegenerative Disease Initiative Plasmid Collection

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   ...types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens in a new...

9. Impact of Genomic Variation on Function (IGVF) Consortium Collection

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   ...their plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources...

10. KLF Research Plasmids

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    ...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

11. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

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    ...identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218 Zhang...

12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

13. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

14. Hot Plasmids: Summer 2024

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    Blog Post

    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

15. Cancer Research Plasmids and Resources

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    ... Labs containing wild-type and mutant alleles described in the initial Target Accelerator publications...

16. AAV Viral Preps

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    ...comprehensive high-quality quality control process includes titering, purity assessment, sterility testing...

17. Fluorescent Protein Guide: In Vivo Imaging

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    ... are highly absorbed by hemoglobin and cause undesirable background signals. Near-infrared fluorescent...

18. Plasmid Collections

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    ...together based on type or application. Browse our guides to find the plasmids and science information you...

19. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

    ... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...

20. Plasmids 101: Restriction Cloning

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    Blog Post

    ...single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert...the orientation of your insert. You may want to design a diagnostic digest for this purpose. Ideally, ...