We narrowed to 680 results for: RON

1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   ...novel organisms and enzymes from these extreme environments. Gearing up for sample collection at the hot...Navigating challenges: collecting and processing environmental samples Since the high temperatures and unstable...

2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   Blog Post

   ...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (...is effective in sensitive cells such as living neurons, and can be fused to other fluorescent proteins...

3. Summer SciComm Series: Master of SciComm

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   Blog Post

   ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...

4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   ...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...

6. The Importance of High Titer for AAV Transductions

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   ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

7. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...high GC content and hairpin structures, ITRs are prone to replication errors and disruptive mutations and...improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085...

8. K. phaffii: Rising to the Occasion

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   Blog Post

   ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

   ...genes and wanted to express them within a specific neuronal compartment, you could express them individually...Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic...

10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post

    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...