We narrowed to 750 results for: Uty

1. Gamma-Retroviral Vector Guide

   Type

   Guide

   ...particles. However, VSV-G is toxic under long-term, constitutive expression. In order to make stable cell lines...and provide stable integration of the intended therapeutic. While similar to lentiviral vectors, they offer...office can provide more information on your institution's best practices with regard to gamma-retroviral-related...Some newer plasmids use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates...

2. Molecular Biology Reference

   Type

   Guide

   ... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute ..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...

3. Adenovirus Guide

   Type

   Guide

   ...response quickly, which has limited their use in therapeutic applications. However, this has also made them...office can provide more information on your institution's best practices with regard to AdV research. ...for outbreak pathogens . Human Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080...

4. Protocol - How to Streak a Plate

   Type

   Protocol

   ...standard labeling system for all your objects/solutions. Sterilize your lab bench by spraying it down ...

5. Modular Cloning Guide

   Type

   Guide

   ...Expression Paul Freemont 78 plasmids including constitutive promoters, T7 expression, RBS strength variants...Bacterial Expression Marco Trujillo Plasmids for reconstituting the ubiquitination cascades of different organisms...

6. Protocol - Over-Agar Antibiotic Plating

   Type

   Protocol

   ...concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic) E. coli...

7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...Isolating a Monoclonal Cell Population by Limiting Dilution Generate monoclonal cell lines from a polyclonal...

8. Protocol - Bacterial Transformation

   Type

   Protocol

   ...more colonies if you use 1 μl of a 1:5 or 1:10 dilution rather than 1 μl directly....

9. Science Guides

   Type

   Guide

   ...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...

10. Antibody Guide

    Type

    Guide

    ...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...