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We narrowed to 752 results for: Uty

Showing: 741 - 752 of 752 results
  1. Centrifugation

    Type
    Protocol
    ...around the center (see image to right). Even distribution ensures that the centrifuge is balanced. Using...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...
  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...(bottom). Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20 °C. To use, dilute...
  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody of known concentration....labs use unpurified tissue culture supernatant routinely without any issues. Plot the mg/mL versus the ...
  4. Western Blot

    Type
    Protocol
    ...1 Reagent A to Reagent B dilution of the BCA assay. Prepare serial dilutions of the BSA standard that ...
  5. Modular Cloning Guide

    Type
    Guide
    ...Expression Paul Freemont 78 plasmids including constitutive promoters, T7 expression, RBS strength variants...Bacterial Expression Marco Trujillo Plasmids for reconstituting the ubiquitination cascades of different organisms...
  6. Molecular Biology Reference

    Type
    Guide
    ... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...Sanger sequencing (Figure 4). Like Sanger, NGS utilizes modified, fluorescently-tagged nucleotides. During...
  7. Science Guides

    Type
    Guide
    ...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...
  8. Antibody Guide

    Type
    Guide
    ...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...
Showing: 741 - 752 of 752 results