We narrowed to 680 results for: RON

1. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

2. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

3. Pushing the Limits of DNA Assembly

   Type

   Blog Post

   ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...

4. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   ...genes and wanted to express them within a specific neuronal compartment, you could express them individually...Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic...

5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

   Type

   Blog Post

   ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...

6. Addgene Depositors Get More Citations

   Type

   Blog Post

   ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...

7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

8. Management for Scientists: Delegating is Key

   Type

   Blog Post

   ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   ...Transformation is the uptake of genetic material from the environment by bacterial cells. In nature, this genetic material...plasmid DNA or fragmented DNA released into the environment. Various factors promote natural transformation...

10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    ...improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without...Recently, the Sidhu group at the University of Toronto expanded the specificity landscape of the SH3 proteins...