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Showing: 761 - 768 of 768 results
  1. Sequencing Primers

    Type
    Guide
    ... along with a more complete list of common sequencing primers. These lists are provided for your reference...Find a list of universal sequencing primers and other common sequencing primers. Educational...sequence verification over the years. Below is a list of popular universal primers available from most...find your plasmid’s page and see what primers are listed under "5' sequencing primer" and "3' sequencing...
  2. Molecular Biology Reference

    Type
    Guide
    ...the insert or other regions of the plasmid. For a list of commonly used primers, check out our sequencing...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...-related information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New ...Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK ...
  3. Modular Cloning Guide

    Type
    Guide
    ...Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids to extend the MoClo-YTK (Dueber) for... Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing highly-tunable GPCR-based... standards. CyanoGate Kit Bacterial Expression Alistair McCormick Kit containing 96 parts and acceptor...Komagataeibacter Tool Kit (KTK) Bacterial Expression Tom Ellis DNA parts to create multigene constructs expressed...
  4. Guide to Using Pooled Libraries

    Type
    Guide
    ...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...cells Sequence those cells that pass (“win”) Get a list of genes involved in the selection NEGATIVE screen...between experimental and control cells Generate a list of gRNAs that disappear with addition of the selection...
  5. Optogenetics Guide

    Type
    Guide
    ...depression, drug addiction, and more. Below is a list of commonly used microbial opsins, including a brief...each and some examples of relevant variants. This list is not exhaustive — please browse our Optogenetics...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...
  6. Educational Resources

    Type
    Guide
    ...using Addgene’s materials, and career advice. Playlists include: Protocols Careers How-to Blog Read about...
  7. Molecular Cloning Techniques

    Type
    Guide
    ...ends can still be fused in a seamless manner. To accomplish this, you can transform into yeast the fragments...
  8. Lentiviral Vector Guide

    Type
    Guide
    ...PMID: 36348415 Stewart, S. A., Dykxhoorn, D. M., Palliser, D., Mizuno, H., Yu, E. Y., An, D. S., Sabatini...
Showing: 761 - 768 of 768 results