...DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ...

...about each library can be found on the individual library page. Find Pooled Libraries of Interest...pneumoniae Veening N/A 1 1,499 MusCK Library 174196 (Library A) 174197 (Library B) 1000000178 (A & B) Knockout... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...experiment, the next step is to pick a library. The pooled CRISPR libraries in Addgene’s collection differ based...a given CRISPR library are unique to the genome of particular organism, and the library is only compatible...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

...Amplifying and Using the Library Once you receive your library from Addgene, check the library information to see...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: ...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid...

...paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest...

...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone...In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses...Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved...Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 ...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting...Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502...

...-Cas9 genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823...

...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

...increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple...

... Data repository From the Frederick National Library for Cancer Research Human Luekocyte Differentiation...

...Pooled Library 111704 Mode #1 Library Pooled Library 111705 Mode #2 Library (14-3-3β) Pooled Library 111706...Mode #2 Library (14-3-3σ) Pooled Library 111707 Mode #2 Library (NEDD4 WW2 domain) Pooled Library 111708...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188531...Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533 iSPI_pSer_Subpool#8 Pooled Library 188534...iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool#10 Pooled Library 188536 iSPI_pSer_Full_library Strain 68306...phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite library ( 111704...

...Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful tools for genetic...experiments. These libraries target the solute carrier family of proteins. Library ID PI Description Human... Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...proteins. Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins...proteins. Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins... Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...Transporter Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human ...

...PHP.B was created by first generating a diverse library of AAV9 mutants that had a small randomized peptide... loop inserted in the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains...

...to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual genes... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...

...prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing...

... coming soon! We’ll also be building our video library all summer long, with additions to our other video...

...for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening ...

...TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library The MORF library is a...Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive collection of human...identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218 Zhang... vectors. The individual clones, or the pooled library containing the entire pooled collection, can be...isoform in pooled screens. This collection and library can be used for screening transcription factors...

...allowing researchers to track and share their own libraries and offering another way to consider and quantify...

...oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier Lab RAS Pathway Clone...and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root labs for characterization...and tumor suppressor. Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator Plasmids...Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance...

...understand their functions. Read More Pooled Libraries Pooled libraries are single preparations of many different...given library have the same backbone, but they express or target different genes. Some libraries cover...Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune...

... to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries - Libraries of Spike (S) Ectodomain...for detection assays and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research...Collection page. SARS-CoV-2 Pooled Libraries - Yeast surface display libraries of Spike (S) Ectodomain and RBD...Receptor Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations for comprehensive...Binding Domain Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein RBD mutations for...full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based activity reporter of SARS-CoV-... SARS-CoV-2 and COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access...

...version,” she said. “If we learn how to make the library even better, we would be very happy to share updated...

...transformation (for example, if you are working with a library or an especially low concentration of plasmid),...

...Pathways & ORFs Synthetic Biology Pooled Libraries Pooled libraries are a set of plasmids all built with ...region. Click here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, ...

... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population of..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...

... Your experiment involves making a large sgRNA library to thousands of genes and targeting and sequencing...clonal differences. If you are performing CRISPR library screens, the same principle holds true - the higher...

...retroviral vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1...propagation of large plasmids, ligated DNA, and libraries, nalidixic acid resistant.  endA1 glnV44 recA1...

...editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of these technologies were without controversy...

...Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for...genome-wide human SAM library SAM can be combined with a human genome-wide library to activate all known...10.1038/nmeth.3047. PubMed . Return to GeCKO Library SAM Library Genome-scale transcriptional activation by... performing genome-wide knockout screens. The libraries are available in two formats: 1 vector system ...lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR page for more information. Full references...transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM...more information, please visit the Addgene SAM library page . Targeted gene activation using SAM There...

...Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...