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Showing: 61 - 80 of 847 results

  1. Cre-lox system

    Type
    Collection
    ...fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ ...optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special...rates of Cre-driven recombination. CREM (or Cre-M) has been engineered to contain an intron, preventing ...

  2. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...sequence can be cloned into BbsI site. gRNA scaffold has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM...

  3. Lentivirus Plasmids

    Type
    Collection
    ...virus integrates randomly into the host genome, it has the potential for insertional mutagensis. The 2nd...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you... that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... can be used in cells or organisms in which Cas9 has been integrated into the genome. gRNA Empty Vectors...Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis...

  7. Rett Syndrome

    Type
    Collection
    ...Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to...

  8. Plan Your Experiment

    Type
    Collection
    ...NHEJ. How do you determine that your desired edit has occurred? The exact method necessary to validate ...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ... extra part, one part has half of the antibiotic gene and the adjacent part has the other half. Any colonies...fully ligated double-stranded DNA molecule. This has proven to be an efficient and effective method for... to improve accuracy and efficiency. Each enzyme has a specific and unique function for the reaction: ...also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool (Link...

  12. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap over ...sit at room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry... loading and allows you to gauge how far the DNA has migrated; 2) it contains a high percentage of glycerol...with water to even out the staining after the gel has been run, just as you would if you had not added ...water and destain for 5 mins. Using any device that has UV light, visualize your DNA fragments. The fragments...

  13. Weighing Reagents Protocol

    Type
    Protocol
    ...weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above...determine if this is best. Check that the balance has the correct capacity for the material that you are...

  15. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...protective measures for each level, as each level has different safety requirements. BSL-1 is designated...is important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository...

  16. Centrifugation

    Type
    Protocol
    ...not require specific PPE, you never know what else has been placed in the centrifuge before you and may ...the centrifuge’s internal lid on the rotor (if it has one) and then close the external lid. Adjust the ...

  17. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...the future you can thaw a new tube that you know has only been thawed once before. Always do controls....want to see is that your vector + insert ligation has many more colonies than your vector alone ligation...

  18. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...starting this procedure, be sure that the cell pool has been sufficiently selected with the appropriate antibiotic... antibiotic so that every cell in the culture has, in theory, been transduced. There are various schools...regularly for transgene expression. This protocol has been optimized for adherent cells but can be modified...

  19. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...). Pro-Tip Add protease inhibitors to 1X if this has not already been done. We typically start with about... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. If the sample ... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. Gently transfer...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-...
Showing: 61 - 80 of 847 results