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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 858 results for: HA

Showing: 61 - 80 of 858 results

  1. Brain Armamentarium

    Type
    Collection
    ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

  2. Retrograde AAV viral preps

    Type
    Collection
    ...Activator DREADD Bryan Roth 121539 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie ...

  3. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...

  4. Bacterial Expression Systems

    Type
    Collection
    ...include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, ...

  6. Plan Your Experiment

    Type
    Collection
    ...contain your desired edit. Each of these methods has their own considerations. Which one to use will come...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ... extra part, one part has half of the antibiotic gene and the adjacent part has the other half. Any colonies...fully ligated double-stranded DNA molecule. This has proven to be an efficient and effective method for... to improve accuracy and efficiency. Each enzyme has a specific and unique function for the reaction: ...also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool (Link...

  8. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap over ...sit at room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry... loading and allows you to gauge how far the DNA has migrated; 2) it contains a high percentage of glycerol...with water to even out the staining after the gel has been run, just as you would if you had not added ...water and destain for 5 mins. Using any device that has UV light, visualize your DNA fragments. The fragments...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above...determine if this is best. Check that the balance has the correct capacity for the material that you are...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...protective measures for each level, as each level has different safety requirements. BSL-1 is designated...is important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository...

  12. Centrifugation

    Type
    Protocol
    ...not require specific PPE, you never know what else has been placed in the centrifuge before you and may ...the centrifuge’s internal lid on the rotor (if it has one) and then close the external lid. Adjust the ...

  13. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...the future you can thaw a new tube that you know has only been thawed once before. Always do controls....want to see is that your vector + insert ligation has many more colonies than your vector alone ligation...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...starting this procedure, be sure that the cell pool has been sufficiently selected with the appropriate antibiotic... antibiotic so that every cell in the culture has, in theory, been transduced. There are various schools...regularly for transgene expression. This protocol has been optimized for adherent cells but can be modified...

  15. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...). Pro-Tip Add protease inhibitors to 1X if this has not already been done. We typically start with about... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. If the sample ... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. Gently transfer...

  16. Cloning

    Type
    Guide
    ...DNA that already has restriction sites on either side of it into any plasmid that has the same sites in...clonase (creating an entry clone). The entry clone now has recombined attL sites flanking your DNA fragment ...fragment of interest. Now that your DNA fragment has been cloned into a donor plasmid, it can be rapidly shuttled...into a donor plasmid (or acquire one that already has your gene into it) and then using bacterial recombination..., such as restriction cloning, once your project has been completed via gateway cloning. Back to Top TOPO...pre-cut, linear, cloning ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase...base pairs. Anything shorter than 200 base pairs has the potential to be completely chewed back by the...

  17. Adenovirus Guide

    Type
    Guide
    ...response quickly, which has limited their use in therapeutic applications. However, this has also made them the...the human population has been exposed to at least one human AdV serotype and has developed a serotype-...immune response has become a disadvantage for the use of rAdV vectors in gene therapy but has, however, proven... digest patterns to determine that the transgene has been inserted into the adenoviral backbone plasmid...other immune-modulatory molecules. Another strategy has been to use oncolytic rAdV vectors, which are replication-competent... of spontaneous reversion to RCAs, although this has been reduced by providing the E1 gene in trans within... adenovirus (RCA) Mutated adenoviral vector that has the ability to replicate and reproduce within host...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...genome can occur, although at a low frequency which has been estimated to range from 0.01% to 3% depending... well-characterized and extensively studied, and has a broad tissue tropism. Table 1 gives a summary of...across a broad range of cell types in vivo. AAV-DJ has been optimized further for specific cell and tissue... tropism towards specific cell types of interest has been to create and test capsid libraries with random...systemic capsids. A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous...naturally cross the BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that ...preps . AAV retrograde (AAVrg) Directed evolution has also been employed to engineer an rAAV serotype variant...

  20. DNA Quantification

    Type
    Protocol
    ... it is possible that the difference between them has affected the accuracy of the NanoDrop. It is a good...
Showing: 61 - 80 of 858 results