We narrowed to 537 results for: PAN

... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...Verdoes Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against zebrafish...

...Institute The Coronavirus Disease-2019 (COVID-19) pandemic was caused by a novel virus strain from the Coronaviridae... The global research community moved quickly to expand the knowledge and understanding of COVID-19 and...

...mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept...the Database Center for Life Science (DBCLS) in Japan. Developed by the Kumiko Ui-Tei Lab . CRISPick (...

...Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature...Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning...

...Genome Engineering You may also like… Genetic Code Expansion Collection Microbiology Plasmids Collection Synthetic...molecule) Escherichia coli Cynthia Collins 112197 pANY3 Temperature (42 °C) Escherichia coli Yingfeng An...

... confluent, expand into larger vessels. A confluent well of a 6-well dish can be expanded into a 10 cm...reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable cell lines Equipment Class II...undergo additional selection while the population expands. At this time, some researchers reduce the concentration...cm dish. A confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method...populations are growing well and have been sufficiently expanded, prepare cell stocks and/or harvest to test for...will have higher levels of expression. Consider expanding populations transduced with a variety of dilutions...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9...

... Monitor cells for growth and expand cells Day 14–30: Analyze and expand monoclonal lines of interest ...cells have expanded but before they become over-confluent, trypsinize the cells and expand them to larger...After the monoclonal lines have been sufficiently expanded, screen the lines for transgene expression and...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9... in individual wells of a 96-well plate after expansion of single cells for 13 days. Note that growth ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

...). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...). You can use either Isopropanol or lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol was adapted...decontamination solution like RNase AWAY® or RNaseZAP® Isopropanol (for precipitation step, Option A) 7.5 M Lithium...volume of Isopropanol to the extracted aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option...

...bacterial cultures , please see those pages. Several companies, such as (Link opens in a new window) NEB , (Link...phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate...plasmid-containing supernatant. Add either ethanol or isopropanol to precipitate the plasmid DNA. Either spin to...for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded RNA...cold 100% ethanol or 350 μL room temperature isopropanol to the solution to precipitate the plasmid DNA...

...containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension/trypan blue mix into....45 µm PES filtering system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium...flask 5–10 times before sampling. Transfer 10 µL of trypan blue into a clean microcentrifuge tube. Vortex ...

...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...

...locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation (ChIP) to allow ...

... CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For enhanced transduction ...

...Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression...

...several modifications to the the pX458 plasmid to expand its usefulness for the lab's gene regulation and...

...CRISPR technologies are continously developing and expanding. Addgene works with leading scientists in the ...

...been completed at the time of shipment. If any discrepancies are identified during this analysis, you will...

...Toolkit Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that...

...or to confirm expected labeling across a tissue panel in one of the antibody’s recommended applications...

... and OZ600 OPEN CODA aATCTTCCACAGTGATGAGGACGTGc panx2 (B1P2E8_DANRE) OZ601 and OZ602 CODA OPEN tGCCCTCTGCCGCCCTGTGGTAGCAg...