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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 533 results for: PAN

Showing: 61 - 80 of 533 results

  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... confluent, expand into larger vessels. A confluent well of a 6-well dish can be expanded into a 10 cm...reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable cell lines Equipment Class II...undergo additional selection while the population expands. At this time, some researchers reduce the concentration...cm dish. A confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method...populations are growing well and have been sufficiently expanded, prepare cell stocks and/or harvest to test for...will have higher levels of expression. Consider expanding populations transduced with a variety of dilutions...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... Monitor cells for growth and expand cells Day 14–30: Analyze and expand monoclonal lines of interest ...cells have expanded but before they become over-confluent, trypsinize the cells and expand them to larger...After the monoclonal lines have been sufficiently expanded, screen the lines for transgene expression and...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9... in individual wells of a 96-well plate after expansion of single cells for 13 days. Note that growth ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...). You can use either Isopropanol or lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol was adapted...decontamination solution like RNase AWAY® or RNaseZAP® Isopropanol (for precipitation step, Option A) 7.5 M Lithium...volume of Isopropanol to the extracted aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option...

  4. CRISPR References and Information

    Type
    Collection
    ...mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept...the Database Center for Life Science (DBCLS) in Japan. Developed by the Kumiko Ui-Tei Lab . CRISPick (...

  5. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature...Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Genome Engineering You may also like… Genetic Code Expansion Collection Microbiology Plasmids Collection Synthetic...molecule) Escherichia coli Cynthia Collins 112197 pANY3 Temperature (42 °C) Escherichia coli Yingfeng An...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures , please see those pages. Several companies, such as (Link opens in a new window) NEB , (Link...phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate...plasmid-containing supernatant. Add either ethanol or isopropanol to precipitate the plasmid DNA. Either spin to...for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded RNA...cold 100% ethanol or 350 μL room temperature isopropanol to the solution to precipitate the plasmid DNA...

  8. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension/trypan blue mix into....45 µm PES filtering system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium...flask 5–10 times before sampling. Transfer 10 µL of trypan blue into a clean microcentrifuge tube. Vortex ...

  12. AAV Viral Preps

    Type
    Collection
    ... CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For enhanced transduction ...

  13. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...

  14. Plasmid Collections

    Type
    Collection
    ...Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...several modifications to the the pX458 plasmid to expand its usefulness for the lab's gene regulation and...

  16. Video Library

    Type
    Protocol
    ...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific... offers career advice for scientists looking to expand their international reach. Joel McDade Joel McDade...

  17. Resolute Plasmid Collection

    Type
    Collection
    ... research in small biotech and pharmaceutical companies. The RESOLUTE project has received funding from...

  18. CRISPR Plasmids and Resources

    Type
    Collection
    ...CRISPR technologies are continously developing and expanding. Addgene works with leading scientists in the ...

  20. Protocol - Bacterial Transformation

    Type
    Protocol
    ...commonly referred to as 'competent cells.' Many companies sell competent cells, which come frozen and are... You should also add a positive control (many companies include a positive control plasmid with their ...
Showing: 61 - 80 of 533 results