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We narrowed to 749 results for: REV

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Showing: 61 - 80 of 749 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...most popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To ...VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion ...for tetracycline-dependent repression led to a reverse Tet repressor, or rTetR, which specifically binds...the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was...system, even though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger...Technology (Link opens in a new window) Content last reviewed 20 June 2025. Do you have suggestions for other...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors...Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct ... and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast...InvivoGen trademark. Return to top Content last reviewed 28 May 2025. Do you have suggestions for other...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371... fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002...selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186...24161108 (Link opens in a new window) Content last reviewed: 12 August 2025...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ... plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed...FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  5. Chemogenetics Guide

    Type
    Guide
    ...Neuronal Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511...brain functions. Annu Rev Neurosci, 37 , 387–407. https://doi.org/10.1146/annurev-neuro-071013-014048 PMID...alternatives for experiments. Perlapine has been previously used in human populations in Japan, making it...Bonci, A., & Michaelides, M. (2017). Chemogenetics revealed: DREADD occupancy and activation via converted...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. Other tyrosine recombinases include... the region of DNA between the target sites is reversed. B) If the target sites are on the same DNA strand...cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...Tracing CRISPR Plasmids and Resources Content last reviewed on 31 July 2025. Do you have suggestions for other...

  7. Antibody Guide

    Type
    Collection
    ...Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus... in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal antibodies only...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...should be run with: a positive control, a sample previously verified to contain your protein of interest ...interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform downstream...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...2009. PubMed PMID: 19633652 Court, et al. Annu Rev Genet. 2002. PubMed PMID: 12429697 Lajoie, et al...this screen the authors were able to identify 7 previously unsuspected genes with known roles in ER-associated... fitness-related genes than have been found in previous screens of this type. They were also able to functionally...backward compatible with all TAL effector scaffolds previously constructed for the Golden Gate TALEN and TAL..., SnoopTag/SnoopCatcher, that can be used to irreversibly link desired proteins together via a spontaneous...isopeptide bond. When used in combination with their previously described SpyTag/SpyCatcher pair, multiple protein... industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production of their products...

  9. Microbiology Resources

    Type
    Collection
    ... S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab...color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...

  11. Plan Your Experiment

    Type
    Collection
    ...RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window...the gRNA vector you have chosen, so it is best to review the protocol associated with the specific plasmid...doi.org/10.1038/nbt.3081 PMID: 25357182 Content last reviewed on 11 July 2025....

  12. CRISPR Plasmids - Parasites

    Type
    Collection
    ...with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites. Moreover...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  13. Mammalian RNAi Tools

    Type
    Collection
    ...Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746–759. PubMed (Link opens in a new window..., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism, ...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level 3 (BSL-3) agent... Plasmid Collection Return to top Content last reviewed on 01 October 2025. Do you have suggestions for...

  16. Recombinases AAV Preps

    Type
    Collection
    ... PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui...

  17. Antibody Production

    Type
    Collection
    ...Usage” and “Delivery” conditions, so we recommend reviewing the specific antibody webpage for further information...the experiment is performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived...

  18. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Specific genes have been mutated or deleted to prevent uncontrolled viral replication, and the required...Topics Page Viral Vectors 101 eBook Content last reviewed: 5 August 2025...

  19. Retrovirus Plasmids

    Type
    Collection
    ...viral genome encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral...101 eBook Biosafety Resource Guide Content last reviewed: 23 October 2025 Do you have suggestions for other...

  20. Promega Plasmid Collection

    Type
    Collection
    ...competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion protein ...NanoBRET® are Promega trademarks. Content last reviewed on: 15 July 2025...
Showing: 61 - 80 of 749 results