...or not, without a guaranteed titer. Any titer expectations communicated by email will supersede the general...after you submit your request, so we can set expectations to the best of our knowledge from the start....not, with a longer turnaround time. Any timing expectations communicated by email will supersede the general...material received. Shipping While Addgene assists importation by including thorough shipping documents and ...taxes, and to complete any additional customs documentation that might be required by your country for the...

...loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

...continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent...adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc...This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease... cell division, all of which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

...Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations for comprehensive...Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free access articles...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Libraries - Libraries of variant Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler ...

...CRISPResso CRISPResso is a robust and easy-to-use computational pipeline that enables accurate quantification...cleavage position to the position of the observed mutations. Coding sequence/s may be provided to quantify...quantify frameshift and potential splice site mutations. The CRISPResso suite accommodates single or pooled ...2.0 From the Church Lab : a tool that identifies putative target sites for S. pyogenes Cas9, S. thermophilus...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38), mouse...

... iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein interactions... Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604...28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643...5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690...

...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...shared experience for the research community and facilitates scientific collaborations between institutions...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

...minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation is to...Addgene will also be speaking or leading workshops. Tentative Meeting Agenda Thursday, September 20 6:00 PM-...how to best help contribute to this publication. Citation information for openly shared communication: Adeno-Associated...

... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

...of enhancers and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage... typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for...

...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge...RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites... to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth Jesse Rinehart 197655...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

...steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation letter DNA...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...

...(Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...resource composed by a collection of databases, computational and experimental resources that relate to the...

... create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik...recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9...

...GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate...regulating the growth of the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively...PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal cancer. Zenonos K...

...dominant manner, are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, ... huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins with abnormally...-onset forms, a fraction of these are due to a mutation in one of three different inherited genes: Presenilin...

...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

... delete genes from or introduce new genes and mutations to the bacterial genome itself. Manipulating the...to study the effects of protein expression in stationary phase. In these cases you may want to control...polymerase subunit ω. Transcription factor binding to a putative promoter region cloned into pH3U3-zif268 (omega...