We narrowed to 157 results for: itch
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TypeBlog Post...importantly, you really are selling yourself. A sales pitch that requires reading two pages or even a paragraph...paragraph to get to the punch line is a terrible sales pitch. State in the first sentence or two what your research...
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Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...from this kit include the production of a suicide switch in cyanobacteria, synthetic inverters in E. coli...biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis ... -
CRISPR History and Development for Genome Engineering
TypeCollection...researchers have begun to use anti-CRISPRs as off-switches for CRISPR genomic editing. Genome Engineering...KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9. Cell . 167(7):1829-1838. PMID... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...photostability, and most importantly, a photoswitching ability. This photoswitching refers to the ability to ‘toggle... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ... -
Technical Design of a Western Blot
TypeBlog Post...relatively cheap and easy to source, making it easy to switch from one readout method to another or change primary...’ll have to use different concentrations if you switch from one to the other. Type of gel The type of... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids... -
Synthetic Biology - Overview
TypeCollection...Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...always learning and that means it is possible to switch your field.” Abigail was also impressed with the... -
Antibody Plasmid Collection
TypeCollection... secrete antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional... -
mTOR Pathway
TypeCollection...nutritional state of the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog Post...Resources on the Addgene Blog Learn about photoswitchable kinases Learn more about fluorescent biosensors... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...Quantitation by Kate Harten DeMaio We made the switch to droplet digital PCR to quantify AAV titers. ... -
Let There Be LITE Plasmids
TypeBlog Post...or off, literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post...For instance, you might use it as part of a flex switch you’d like to work with in neurons or you might... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle... -
Editor's Choice, August 2016
TypeBlog Post...Management Software for Biology Adapting Toehold Switches to Detect Zika Virus As always, if you have any... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...deadlines, and sync everything to your calendar. Ditch the paper To Do List and move to one that moves ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...