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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 845 results for: tat

Showing: 61 - 80 of 845 results

  1. COVID-19 Resources

    Type
    Collection
    ...Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations for comprehensive...Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free access articles...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Libraries - Libraries of variant Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler ...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...

  3. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein interactions... Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604...28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643...5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690...

  4. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...shared experience for the research community and facilitates scientific collaborations between institutions...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein ...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain...important for tetracycline-dependent repression. Mutating these residues led to a reverse Tet repressor,... lineages and transcriptional and proliferative state of each cell in a population. Proliferation history...tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity . Proc Natl...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    ...of enhancers and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage... typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for...

  8. Genetic Code Expansion

    Type
    Collection
    ...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge...RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites... to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth Jesse Rinehart 197655...

  9. CRISPR References and Information

    Type
    Collection
    ...CRISPResso CRISPResso2 is a robust and easy-to-use computational pipeline for the analysis of genome editing ...experiments. It enables rapid and intuitive interpretation of results produced by amplicon sequencing.... opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38), mouse...

  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

  12. AAV Packaged on Request

    Type
    Collection
    ...steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation letter DNA...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...

  13. Plant Plasmids and Resources

    Type
    Collection
    ...(Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...resource composed by a collection of databases, computational and experimental resources that relate to the...

  14. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik...recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9...

  15. Ras Pathway

    Type
    Collection
    ...GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate...regulating the growth of the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively...PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal cancer. Zenonos K...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...dominant manner, are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, ... huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins with abnormally...-onset forms, a fraction of these are due to a mutation in one of three different inherited genes: Presenilin...

  17. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...
Showing: 61 - 80 of 845 results