...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...Insert Promoter Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker...

...accessible toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant...blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant...

...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

...CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation...targeted strand non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary...

...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...

...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker...

... length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details...two-hybrid reporter system but have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene ...

...microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...

...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing Genomes - Cold...

...DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find... Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions, but what about...

...interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S, ...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter PI Publication ...

... procedures. Additionally, because this virus integrates randomly into the host genome, it has the potential...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

...Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base edit human...Alzheimer’s disease and related disorders. The site curates information on useful resources such as: Antibodies...

...-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397 (Link... Endy and Philippa Marrack Labs. Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Joung ...

...window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms are reversible in...Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

...Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes,...residual ethanol wash and add RNase-free water or TE as soon as the entire tube is dried but while the...visible. Resuspend RNA pellet in RNase-free water or TE. Quantify and assess the quality of your RNA sample...Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000 × g at 4°C ...Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000 × g at 4°C to separate...

...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

...Reddy TE, Gersbach CA (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes... M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA (2017) CRISPR–Cas9 epigenome editing...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes...-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted...transcription. Created with BioRender.com. Tet1 initiates cytosine demethylation of DNA. However, several...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...