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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 964 results for: tes

Showing: 61 - 80 of 964 results

  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details...two-hybrid reporter system but have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene ...

  2. Microbiology Resources

    Type
    Collection
    ...microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...

  3. Worm Expression Resources

    Type
    Collection
    ...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing Genomes - Cold...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter PI Publication ...

  5. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...transfer vectors you can find reporter plasmids to test whether or not you've efficiently generated infectious...recombinases to remove genes flanked by either loxP or FRT sites respectively, and empty vectors to express your ...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base edit human...Alzheimer’s disease and related disorders. The site curates information on useful resources such as: Antibodies...

  7. Rett Syndrome

    Type
    Collection
    ...window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms are reversible in...Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

  9. Retrograde AAV viral preps

    Type
    Collection
    ...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

  10. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find... Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions, but what about...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ...tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence of tetracycline...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes,...residual ethanol wash and add RNase-free water or TE as soon as the entire tube is dried but while the...visible. Resuspend RNA pellet in RNase-free water or TE. Quantify and assess the quality of your RNA sample...Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000 × g at 4°C ...Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000 × g at 4°C to separate...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Reddy TE, Gersbach CA (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes... M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA (2017) CRISPR–Cas9 epigenome editing...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes...-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted...transcription. Created with BioRender.com. Tet1 initiates cytosine demethylation of DNA. However, several...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Thakore PI, Perez-Pinera P, Brown MT, Majoros WH, Reddy TE, Gersbach CA. Correction of dystrophin expression...Ousterout DG, Kabadi AM, Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome ...grip strength, specific force, and other muscle tests, muscle function is clearly improved, although not...at the ten highest-ranking predicted off-target sites. Each paper characterizes additional, unique facets...

  15. Neuroscience

    Type
    Collection
    ... represent the official views of the National Institutes of Health....

  17. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

  18. Antibodies

    Type
    Collection
    ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

  19. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...
Showing: 61 - 80 of 964 results