We narrowed to 955 results for: tes

...untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ...tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence of tetracycline...

... represent the official views of the National Institutes of Health....

...and terminators repressors and activators logic gates Networks and Gene Regulation Plasmids Search the...

...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

...in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...

...plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available...

...specific genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and ...

...requires delivery of hundreds of gRNAs with target sites throughout the chromosome. Figure 1: Overview of...

...integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry and sfgfp...

... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...

...DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for use! Plasmid...centrifugation and washed before DNA is eluted with water or TE. The Bishop Lab’s glass milk protocol is adapted ...than 30 minutes and only requires incubating the columns with 1M phosphoric acid for 3 minutes followed...syringe-based method can be completed in 20 to 30 minutes. Additionally, while commercial plasmid prep kits...same as those provided with the kit. Although not tested by the authors, it’s possible that used plasmid... the National Academy of Sciences of the United States of America, 76 2, 615-9 . https://doi.org/10.1073...

...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

...shared experience for the research community and facilitates scientific collaborations between institutions...

...transgene under CMV Bamburg 16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb...

... μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100%...100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized...the column using water or a neutral buffer such as TE. You will now have plasmid DNA that has been purified... Water-saturated phenol-chloroform can be used if TE-saturated is not available. Vortex microfuge tube... paper towel for 5-20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension...min. Centrifuge the tube for 5 min at 12,000 g. Notes: Pellet contains proteins, cell fragments, salt ...