We narrowed to 810 results for: mal

...Use this protocol to transfect mammalian cells with your plasmid of interest....protocol describes a general method for transfecting mammalian cells using linear polyethylenimine. Transfections...are below passage 20 for viral production. The optimal mass DNA:mass PEI ratio will need to be empirically...

...These tools allow you to observe specimens much smaller than you would be able to with your naked eye, ...advantage of the physical properties of light to detect small objects. Two of the most important properties of...needed, you can also use the fine focus knob (the smaller of the focus knobs) to make minor adjustments to...

...). You might need to express YGOI in cultured mammalian cells. The problem is that the only version of...Using subcloning, you can easily move YGOI into a mammalian expression vector. Design (Choosing enzymes) Many...purpose. Congratulations, you now have YGOI in a mammalian expression vector and can begin your studies. ...

...transfection. Some lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin...cycles. Procedure Before beginning, determine the optimal dose of selective reagent for your target cell ...important to do regular media changes and maintain optimal growth conditions for the surviving cells. Even...

...internal passive reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and ... a validated standard curve is obtained, make a small aliquot of each standard (enough for 1 or 2 plates...acceptable) Baseline removal: all samples will have some small amount of background signal that is most evident...

...medium with increased serum concentrations, may be optimal for different cell types. Before starting this ...because the buildup of waste products could be suboptimal for cell growth. This conditioned medium will...10 5 cells/mL) = 0.125 µL Because this is such a small volume, first make 1 mL of a 1:100 dilution of the...

...risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes prior...302411100 Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad...plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling...

...accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing out an amount...amount of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may...

...pathways. Receptors are remotely controlled via small molecules, which allows for specific control of ...

...sterile water (instead of TE buffer) to ensure optimal salt concentrations in subsequent reactions. Step...per annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

...time (approximately 18–30 h). On the other hand, smaller plasmids can be present in large numbers, 50 or...may help to increase the density of the culture. Normally cultures shake at 150–250 rpm, increase this to...

...the bacteria back into suspension, but within a smaller volume of buffer that is compatible with the next... genomic DNA to precipitate, while leaving the smaller plasmids free in solution. Pellet the proteins ...

...Reverse MBP-F GATGAAGCCCTGAAAGACGCGCAG 3' end of maltose binding protein Forward mCherry-F CCCCGTAATGCAGAAGAAGA...

...~220 mL) just in case we spill anything or have small errors in measurement. You should also always make...-mix slightly. Even so, you should always use thermally insulated gloves when removing anything from the...

... Short primers are mainly used for amplifying a small, simple fragment of DNA. On the other hand, a long...

...plasmid DNA prep the following day. Tips and FAQ The optimal concentration of long-term glycerol storage is ...

...toothpicks or wire loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar ...

...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

...-8 pieces such that all (or most) fragments are small enough to be accurately sized on a gel and also ...

...height. They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...