We narrowed to 680 results for: RON
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TypeBlog Post...particularly helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools. References...
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All in a Twist: dsRNA
TypeBlog Post...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...telomeres and centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post... psoriasis Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes... -
RNA Extraction Without A Kit
TypeBlog Post...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...more useful! From FPs optimized for oxidizing environments to photoconvertible variants, it seems like ... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...automate the process as well. Sign up for eTOC (electronic table of contents) alerts from journals you read...useful papers. For many, this collection is purely electronic. Gone are the days of file cabinets filled to... -
Working with Nuclear Receptors
TypeBlog Post...for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins... the genome to sense the impact of the total environment on the cell. The NURSA Consortium Now in its ... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or... Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. doi:10.3389/fpls.2015.01001 Ho... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...