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TypeCollection...Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research Reprogramming Plasmids Differentiation...Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells are proliferative, unspecialized...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used in ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...
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Zhang Lab CRISPR Page
TypeCollection...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab... Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR ...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse...involved in defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination... -
Microbiology Resources
TypeCollection... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene’s repository ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or check ...out our additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...Your Perfect Plasmid Backbone Empty backbones are plasmids containing only the elements needed for replication...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent...marker Also see more empty backbones on our Plant Plasmids and Resources collection page Yeast GAL4, PGK,... -
CRISPR Plasmids - Tagging
TypeCollection...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Several different tagging techniques, as well as the plasmids and protocols needed for using them, are described...Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag (currently...based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair...arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were tested ... -
CRISPR Plasmids and Resources
TypeCollection...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together .... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in... -
Viral Vector Guides and Plasmids
TypeCollection...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are ...more plasmids containing the necessary components of the viral genome to assemble the viral capsids (packaging...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...information to cells. These vectors rely on production plasmids that have been carefully optimized for both safety...vectors and find viral production and transfer plasmids for a wide array of research purposes. Interested... -
Plasmid Collections
TypeCollection...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ...Collections Addgene's repository contains 165,343 plasmids . We've gathered our curated plasmid resources...Expression Worm Expression Kits Kits are groups of plasmids that have been packaged together because they ...Pooled Libraries Pooled libraries are a set of plasmids all built with the same backbone and only differing... -
Fluorescent Protein Guide: Biosensors
TypeCollection...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more. Metal Ions ...Neurosci. 2020 Dec;23(12):1629-1636. Jordane Dimidschstein Calcium Calcium sensor in astrocytes (GCaMP6f...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin ...Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP Biosensor for ADP based on tetramethylrhodamine-labeled... -
CRISPR Guide
TypeCollection...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...archaea use to protect against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety ...CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can ... (see: Validate genetic modification ). Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1...Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3...Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 h Monomer...Emission (nm) Brightness pKa Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1...Emission (nm) Brightness pKa Maturation Structure Plasmids mCrimson3 588 615 Monomer mCrimson3-N1 - Mammalian... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids that provide Cas9...gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas protein act...of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a...single CRISPR function. Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9... -
Synthetic Biology - Overview
TypeCollection...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...components. Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic...level gene networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental...including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian... -
Synthetic Biology - Browse Plasmids
TypeCollection...Collections Synthetic Biology SynBio Plasmids Synthetic Biology: SynBio Plasmids SynBio Resources SynBio Home ...list of available plasmids related to synthetic biology below. If there are plasmids you would like to...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....please email [email protected] . Synthetic Biology Plasmids Search the table by keyword or sort by the table... -
Synthetic Biology - Cloning and Genomic Tools
TypeCollection...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...Synthetic Biology plasmids for cloning and genomic tools. Plasmid...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table... Back to Top Do you have suggestions for other plasmids that should be added to this list? Fill out our... -
Fluorescent Protein Guide
TypeCollection...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty.... Michael Davidson Fluorescent Protein Collection Browse the comprehensive Michael Davidson collection...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists...Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains... biosensors. Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and control molecular... of cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate or inhibit genetically... -
Neurodegeneration Research Collection
TypeCollection...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons.... Lam et al. bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription...APP processing and dynamics using APP and Bace1 plasmids . Capitini et al. iScience. 2023 Apr 11. See More...research community. This collection highlights plasmids created with support from the BRAIN Initiative... -
Michael Davidson Fluorescent Protein Collection
TypeCollection...from the Michael Davidson Lab.... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein...Protein Collection Michael Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald...Orange Red Photoactivatable The lab of Michael Davidson from Florida State University has contributed ...localization data found below. In addition, the Davidson lab has contributed the corresponding empty backbone...proteins with your gene of interest. Most of these plasmids are available to industry scientists as well as... more about this collection, or visit Michael Davidson's Molecular Expressions website (Link opens in ... -
Depositor Collections
TypeCollection...Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors ...expression, synthetic biology, and viral vector plasmids. Plasmid...Collections Addgene's repository contains 165,343 plasmids, which includes plasmid collections from depositors...TREAT-AD Plasmid Collection Visualization Michael Davidson Fluorescent Protein Collection Allen Institute... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...Excitation/Emission Brightness Plasmids mCardinal 604/659 18 mCardinal plasmids from Chu et al. (2014) mNeptune...Find plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins...imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Near-Infrared Protein Excitation/Emission Brightness Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/...Photoactivatable Protein Excitation/Emission Brightness Plasmids PAiRFP1 690/717 (after photoactivation) 3.2 pPAiRFP1... pPAiRFP2-N1 Do you have suggestions for other plasmids that should be added to this list? Fill out our...