We narrowed to 680 results for: RON
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TypeBlog Post...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...
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Magnetic Control of Proteins: More than a Dream
TypeBlog Post...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024...science in the open The York Lab operates with a strong commitment to exploration and collaboration. Andrew... -
Pooled Library Amplifications
TypeBlog Post... library in a lentiviral backbone might be more prone to recombination, while a library with a large backbone...transcription factor overexpression library) or is prone to recombination, we recommend amplification on ... -
Of Myc and Men
TypeBlog Post...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https://doi.org/10.1128... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...the resistance cassette has been placed into an intron. Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd... -
Experimenting with New Careers while in Grad School
TypeBlog Post...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional... -
Genome-wide Screening Using CRISPR
TypeBlog Post... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...