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We narrowed to 1,050 results for: ins

Showing: 901 - 920 of 1050 results

  1. Optogenetics Plasmid Collection

    Type
    Collection
    ...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps...encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins, which...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...expression and localization. Many other types of proteins can also be controlled by light, generally by ...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that have homology... potential target sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids...2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given ...NGG PAM sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized...2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized...SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized...

  4. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse...mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit...

  5. Adenovirus Plasmids

    Type
    Collection
    ...commonly used adenovirus terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description...beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer Vogelstein...modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single...Collection of adenovirus shuttle vectors and strains Viral...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids...database for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1...recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral A ...

  6. Antibody Plasmid Collection

    Type
    Collection
    ... monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount...of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase...desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...Service: AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties ...transfer (FRET) efficiency of a pair of fluorescent proteins. Genetically encoded biosensors can be introduced...Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul...nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...

  10. Lentivirus Plasmids

    Type
    Collection
    ...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights ...1alpha-driven constitutive transgene expression. Contains intron that gives high expression. Didier Trono...and Paul Kaufman 102930 pLJC5-Tmem192-3xHA 3rd Contains a lysosomal tag. See Abu-Remaileh et al. (2017...expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch...and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , vif , and nef . Can be...transfer plasmids. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains gag and pol . Can be used with 3rd generation ...

  11. Plan Your Experiment

    Type
    Collection
    ...small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote...including point mutations, base changes, and large insertions or deletions. For more information on the wide...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their activity...sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations using a prime... In many cases, you can synthesize, anneal, and insert the targeting oligos into plasmids containing the...can generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile...

  12. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Publication Return to top Parasite ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base... been designed for use in Drosophila and other insects. CRISPR... been designed for use in Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...

  15. Distribution to Industry

    Type
    Collection
    ... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity... constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting ...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...role in secreting proteins from the cell to the extracellular environment. Proteins are first translated...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular... (find plasmids for cloning in our Fluorescent Proteins Empty Backbones Collection ). Then, by examining...the same structures as these well-characterized proteins. General Structures Eukaryotic cells contain a...FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime...

  18. Genetic Code Expansion

    Type
    Collection
    ...Bacterial TAG Wei Niu Browse Strains The table below highlights bacteria strains that have been modified to...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for ...genetic code can help us understand and evolve proteins for various purposes from therapeutics to biopolymers...phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic code expansion without buildup of...DE3) ΔserC serC gene knocked out; for expressing proteins containing site-specific non-hydrolyzable phosphoserine...and trpT genes). Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS...

  19. CRISPR Plasmids - Yeast

    Type
    Collection
    ...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base ...the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate...activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...
Showing: 901 - 920 of 1050 results