We narrowed to 1,021 results for: App

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you...

2. Common Injection Routes in Mice

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   ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

3. Anatomy of a Plasmid Page at Addgene

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   ...those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication...

4. Genome-wide Screening Using CRISPR

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   ...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

6. Simplify Cloning with in vivo Assembly

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   ...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

7. CRISPR 101: RNA Editing with Cas13

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   ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

8. Negative Can Be Positive: Open AAV Data with Addgene

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   ...this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

10. DNA Purification Without a Kit

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    ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

11. Plasmids 101: Protein tags

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    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

12. Save Time with Transient Plant Leaf Transformations

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    ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...Often times, a gene gets its name based on what happens to an organism when a gene/protein is depleted ...

14. CRISPR-mediated Plant Base Editors

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    ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

16. Twenty Years of Sharing: Expanding Accessibility

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    ...like viral preps and libraries were kept at the appropriate temperature for the duration of their journey...

17. Plasmids 101: Stringent Regulation of Replication

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    ...carefully regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients...

18. An Integrin Antibody Toolkit from IPI

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    Blog Post

    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate...

19. Filming Science Videos in the Age of Social Distancing

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    ...refining the process down to a place I could be happy with it. Cycle 5 - The Future! Figure 5: ...

20. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...many of the people who work in them are incredibly happy with their jobs. One of the Saturday Hack Day projects...