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We narrowed to 1,026 results for: LEA

Showing: 961 - 980 of 1026 results

  1. Five Popular Model Organisms

    Type
    Blog Post
    ...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviruses has a long incubation period that eventually leads to serious disease and occurs in many species. Some...comparison of second- and third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral...HEK293T cells will produce the viral vectors and release them into the supernatant. After an initial media...stimulates the expression of transgenes via increased nuclear export. RRE in cis Rev Response Element; the sequence...and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid from gag...like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from the Retroviridae...

  4. Modular Cloning Guide

    Type
    Guide
    ...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...BpiI/BbsI to cut outside their recognition site, leaving DNA fragments with an overhang of 4 bp. If each...integrate up to three transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use ...Vladimir Nekrasov 95 plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for..., TALEN Feng Zhang Plasmids for building TALE nucleases (TALENs) and transcription factors (TALE-TFs) ...

  5. Molecular Biology Reference

    Type
    Guide
    ...Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and ...cell division, and each daughter cell receives at least one copy of the plasmid. By the 1970s, the combined... ( ori ), an antibiotic resistance gene, and at least one unique restriction enzyme recognition site (...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...genes, marking the birth of molecular genetics. To learn more about different types of cloning methods, check...of its sequence to design a effective primer. To learn more about the sequencing technologies Addgene uses...

  10. Optogenetics Guide

    Type
    Guide
    ...relevant variants. This list is not exhaustive - please browse our curated list of opsins here to find ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...protein in the light ~400 (on)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and.... The HEK293T cells will produce the virus and release it into the supernatant. After an initial media...integration sites are found in proto-oncogenes. This can lead to activation of the oncogene and possible development...stimulates the expression of transgenes via increased nuclear export. Psi (Ѱ) in cis RNA packaging signal; recognized...like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material (cDNA...

  12. Guide to Using Pooled Libraries

    Type
    Guide
    ...your screen. If you need to amplify the library, please refer to the depositor’s protocol for the best ... library is complete - an incomplete library may lead to false positives or false negatives in later experiments...minimizes the effects of random chance that could lead to false positive or negative results - for a given...sequencing services. For an NGS company near you, please consult these links: NGS Company Resources Science...

  13. Chemogenetics Guide

    Type
    Guide
    ...population they are targeted to. Read this guide to learn more, or explore Addgene's Chemogenetics Plasmid...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...For a discussion of alternative DREADD ligands, please see the text. PSAMs Unlike DREADDs, which manipulate...(Fz) or chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics...

  14. Adenovirus Guide

    Type
    Guide
    ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses... integrated in the packaging cell’s genome could lead to the production of replication-competent adenoviruses... of the human population has been exposed to at least one human AdV serotype and has developed a serotype-specific...like viruses or bacteria, within a host organism, leading to disease. Innate immunity Non-specific immune...

  16. Educational Resources

    Type
    Guide
    ...development. eBooks Download any of our eBooks to learn about popular topics related to our collections....Plasmid Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction...

  17. Science Guides

    Type
    Guide
    ...created the following guides to help you start learning about various scientific concepts related to our...Read More Viral Vectors Find educational guides to learn more about the different types of viruses that are...

  18. Promoters

    Type
    Guide
    ...Learn about bacterial and eukaryotic promoters, including transcription and the transcription complex...phospholycerate kinase gene U6 Constitutive Mammalian U6 nuclear promoter for small RNA expression (species-specific...

  19. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... 37 °C for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded...colonies and to generate liquid bacterial cultures , please see those pages. Several companies, such as (Link... Note: This step causes the bacteria to lyse, releasing their contents, including plasmid DNA, into solution...proteins and genomic DNA to precipitate, while leaving the smaller plasmids free in solution. Pellet the...times to mix the contents. The contents will become clear and thicker as the proteins and DNA are denatured...temperature on the highest setting. Note: You should see clearly separated layers: Top Phase - Aqueous DNA phase...precipitate. Centrifuge solution at high speed (at least 12,000 rpm) for 15-30 min at 4°C. Notes: Pellet ...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ...setting that gets the sample to at least 121 ℃ under 20 psi for at least 30 min. The high pressure will prevent...bath. Pro-Tip You should leave the molten gel-mix in the water bath for at least 5 min. Do not let any of...the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label the bottle...initials, the date, and the bottle contents. This will clear up any confusion later if your forget your bottle...where you’ll be pouring your plates - be sure to leave room for your bottle of molten gel mix, a tube rack...opening the door to the autoclave just a crack and leaving it open that way for ~10 min. This will allow any...microwaving (if you microwave, beware of over-boiling!). Leave your plates out on the bench to solidify. Pro-Tip...
Showing: 961 - 980 of 1026 results