Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 500 results for: RAP

Showing: 81 - 100 of 500 results

  1. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...Some things cannot be photographed or need text to properly contextualize. Graphics/cartoons (no words...be a hard sell!) Digital platforms often have demographic data of their users readily accessible via web...have a solid understanding of their audience demographics. Do your research so you can go to where your...depending on factors like socioeconomic status and geographic location. For example, not all social media platforms... biology are often more suited for cartoons or graphics for visuals. However, it’s much more challenging...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...may need text for audience interpretation. Infographics Low Combines both words and images; especially...

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and ...’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on another...work with dystrophin exon skipping? For such a therapy to be successful, multiple requirements must be...minimal risk of off-target editing. In order for the therapy to persist over time, stem cell editing would be...in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients. ...characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause...represent a great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler...

  3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...discovery/development of genetic targets and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting... may not be the most likely candidate for gene therapy, however, because the disease affects multiple ...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...mutations when using this technology. When CRISPR therapies are being given in vivo, it is difficult to use... References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology.  https://doi....1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...human gene therapy. Molecular Therapy: The Journal of the American Society of Gene Therapy, 14(3), 316...AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice for...high transduction efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel...rAAV treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes...AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 785. https://doi.org...in Mouse and Human Skeletal Muscle. Human Gene Therapy, 31(3–4), 233–240. https://doi.org/10.1089/hum.2019.173...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org... Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169...Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in... in the rat spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434...Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016...corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–74. https://doi.org/10.1038/s41434...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...development of toolkits such as the SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering...unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the need for ...rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to optimize...built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically boosted...repair template of your choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs...editing in other organisms and systems, allowing rapid progress in a wide range of fields.   Thank you...genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from...from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR...choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single...single tube. You can also find additional SapTrap plasmids used for introducing point mutations that was published... Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...of combining targeted molecular therapies for cancer with chemotherapies, or developing innovative mobile...clinical research to discover and test the best new therapeutics. Consultants often work with such teams to help...human resources towards the development of a new therapy based on its market need and anticipated commercial...context of significant clinical uncertainties and a rapidly evolving healthcare space. The consultants who ...at the core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance...and the commercial applications of a particular therapy. With your scientific knowledge behind you, you...business and scientific understanding of successful therapeutics can be a great fit for former healthcare consultants...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction heterodimerization...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP...heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell does... the mitochondria (binding FRB), allowing for a rapid and inducible inactivation. Find the Robinson plasmids...approach to control for these effects, drawing on the rapidly growing field of optogenetics. Using a rational... J 2014 Aug 1;33(15):1713-26. RUSH & CRUSH – rapid & conditional gene silencing in RNAi transgenic ...CRUSH alleles off or on enables this method to rapidly address questions of tissue-specificity and cell...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic applications where longer...antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...better tissue penetration which is useful for therapeutic and imaging applications; and reduced immunogenicity... longer retention times often increase therapeutic efficacy (Ahmed et al., 2012). How do you generate ...Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic (Bates... for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs...Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 34:404–417 . https://doi.org/10.1089/mab.2015.0036...

  11. Five Popular Model Organisms

    Type
    Blog Post
    ...successfully helped researchers develop a variety of therapies. For example, many scientists use mice to study...the complex interactions between cancer cells, therapeutic drugs, and the rest of the body than studies ...mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another...important human disease model with potential for therapeutic drug discovery as it is estimated that 75% of...they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio...elegans are small, transparent worms that have a rapid life cycle and large brood sizes. The majority of...Drosophila melanogaster and the role of the fly in therapeutic drug discovery." Pharmacological reviews 63.2...

  12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...APEX: engineered ascorbate peroxidase that can rapidly biotin label proteins A year after BioID became...  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo...hours to label proteins and APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic...more information, read the Split-BioID blog post.  RaPID: RNA-protein interaction detection So far, we’ve...that can regulate multiple biological functions. RapID (RNA-protein interaction detection) uses proximity...interactions (Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis... compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA* and an RNA component...

  13. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...more hands on While teaching courses about gene therapy and genetic manipulation, I and other professors...similar types of thinking go into developing gene therapy strategies for various diseases. That’s when I ...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...pick the best strategy and propose a final gene therapy approach for the disease to be openly discussed...impression was that they had clearer ideas about gene therapy in the following lessons. And let me tell you more...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps...gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may ...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...lab from the University of Utah developed SapTrap. The SapTrap system uses golden gate assembly to produce...Ras oncogenes have been difficult to target therapeutically due in no small part to the central role Ras...restriction enzyme site and unique primer sites for more rapid screening by restriction digest and Sanger sequencing... sites, this technique also allows for easy and rapid targeting of complex elements to specific genomic...genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing is...(ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations and combinatorial...et al. Metab Eng. 2012. PubMed PMID: 2232647   SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...

  15. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the...Analyze . Select Gels . Select Plot Lanes . One graph per lane will appear with peaks representing each...

  16. Western Blot

    Type
    Protocol
    ...microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody Deionized water...BSA standard concentration versus absorbance. Extrapolate the total protein concentration of the sample...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...organism. These features make AAV ideal for human therapies and in vivo laboratory experiments ranging from...in the nucleus and is a good option if you need rapid transgene expression, since you don’t have to wait...transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958. Vandenberghe...adeno-associated virus manufacturing." Human gene therapy 21.10 (2010): 1251-1257. PubMed PMID: 20649475....Efficient AAV Vector Production System: Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772...the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, ...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5), 785 (2023). PubMed...

  18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...primary human cells. Now, scientists at Beam Therapeutics have found a way to improve editing in primary... and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed bacterial selection...research! Base editing in primary human cells  Cell therapy approaches can use base editors to correct mutations...efficiently makes it a promising tool for T cell therapy development.  Reducing off-target editing in ABE8s...editing specificity, for example for potential therapeutic approaches, the authors recommend mRNA delivery...off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved...adenine base editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org...

  19. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... islandicus CRISPR-Cas system. 5. Phage therapy Phage therapy uses phage to treat bacterial infections...promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity...bacterial pathogens possess CRISPR-Cas systems, phage therapy might not be as effective. This is where Acr proteins...enough that they could be engineered to “armor” therapeutic phages to protect them from pathogenic bacteria...activity is an important step towards CRISPR-based therapies and for more precise editing in the lab. References...Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https...

  20. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... agarose addition, we found it was possible to rapidly punch out cell clones with sterile truncated pipet...from a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60...only warm but not hot- and still liquid- it is rapidly poured onto the middle of the plate, where it immediately...and partially dislodged from the agarose plug by rapid pipetting, as shown in the YouTube video. Before... appear to be yielding well-spaced clones. For rapidly growing cells we sometimes use TPP peelaway T-75...drop of serum to each well in the 48-well plate; rapidly growing cells such as HEK and CHO do not seem to...technique should be useful to scientists who wish to rapidly isolate individual cell clones with different expression...
Showing: 81 - 100 of 500 results