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We narrowed to 311 results for: cas9 genes

Showing: 121 - 150 of 311 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system...2014). PubMed. The Use of CRISPR/Cas9, ZFNs, and TALENs in Generating Site-Specific Genome Alterations...Homology directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world ...the gRNA/PAM site remains intact. In other words, Cas9 will keep cutting and repairing your target DNA ...of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed...editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al...your desired edit are all you need to generate a knock-in.   General considerations for designing a repair...

  2. CRISPR References and Information

    Type
    Collection
    ... sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,... window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA...CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively selected sgRNAs, genes, or...118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a ... 0.001% of the population.  Because indel generation by Cas9 can occur at a steady rate, scientists found...promoters driving expression of Cas9 were used in this study.  The zCas9i cloning kit is a plant cloning...activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12...a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable ...12 guides per gene where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs ...anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein that is smaller and easier to deliver...

  4. Zhang Lab CRISPR Page

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    Collection
    ...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic... of endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA...of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can be...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide...are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent Cas9 mouse Constitutively...cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...isoform-specific gene expression. Gene isoforms refer to mRNAs generated from the same gene but with different...variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to induce gene transcription...isoform-specific gene regulation — at least, more straightforward than generating cDNA for each gene isoform individually...first ask yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for...with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church... neuron-optimized CRISPR/dCas9 activation system for robust and specific gene regulation." eNeuro 6.1 ...transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice." Nature...

  6. CRISPR Guide

    Type
    Collection
    ... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...dual nickases to generate a knockout or gene edit; or using Cas9 to generate large genomic deletions or ...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the most commonly...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...

  7. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ... - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated...the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has ...tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G•C edits... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR... in Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA ...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be ...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied interest in...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...while Class 2 use single protein Cas effectors, like Cas9. The Cascade complex doesn’t usually carry out the...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known...

  9. Plan Your Experiment

    Type
    Collection
    ... RNA delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which...used to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein ...knockin. Select gene and genetic element to be manipulated In order to manipulate a given gene using CRISPR...mutant allele of a gene ( point mutant )? Increase or decrease expression of a target gene? Once you have ...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA targeting the...concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...hammer: Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated...technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter of a target gene. Here, the target window...you are trying to do: gene knockout, a specific base edit, or modulation of gene expression. Location ...multiple sequences targeting a gene to score in order to call that gene a hit.  This is an exciting time...Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https:/...protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are both [5’-20nt-NGG...This means that, for gene editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    ...editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase and a prime editing...., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure...pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created ...along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the newly synthesized...Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA editing...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, ...corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing via ...the CRISPR/Cas9 system to both precisely insert the MECP2-R270X mutation into the MECP2 gene in wild-type...protein 2 (MECP2) gene, Rett Syndrome is a rare, progressive, neurodevelopmental genetic disorder that affects... CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced Pluripotent Stem Cells. Front Genet 10: . https...of Rett Syndrome research, the role of the MECP2 gene, and highlights some of the latest breakthrough ...

  14. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate... Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875 Bacteria BsaI none S. pyogenes Chloramphenicol...meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9/pTREX-n 68708 Other/...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein...cut S. pyogenes mCherry Kuhn pU6-(BbsI)_CBh-Cas9-T2A-BFP 64323 Mammalian BbsI yes, cut S. pyogenes BFP Kuhn...

  15. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty ...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem...NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ... plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication...or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a ...

  16. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410 pCFD3-dU6...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a ...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin..., need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ... plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...critical for Cas9 cleavage), thereby rendering the resulting construct resistant to Cas9 induced cleavage... introduced to endogeneous DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-...resistant to Cas9 cleavage. If a plasmid contains the template, site-directed mutagenesis can be used ...footnote; a similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...relevant in this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate...modifications.   Site-directed mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers...

  18. Zhang Lab's CRISPR Frequently Asked Questions

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    Collection
    ...the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does... engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA has high efficiency...effect). The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, ...can express two different chimeric gRNAs with the Cas9 nickase which will together introduce cleavage of...thorough step-wise instructions on designing and using Cas9-based genome engineering tools to perform different...PAMs in the region, will they also be targeted? The Cas9 cuts 3-4bp upstream of the PAM sequence. There can... can be some off-target DSBs using wildtype Cas9. The degree of off-target effects depends on a number...

  19. CRISPR History and Development for Genome Engineering

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    Collection
    ...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease... anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...capability of Cas9 to make other types of modifications at specific loci. Activate or Repress : dCas9 fused to...

  20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to... tetracycline cas9 inducible knock-out system. His lab is also exploring the use of cas9 proteins from...were identified for 61 such diseases. Now single gene alleles have been identified for 4,163, over 50%...Lowe talked about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments...may be involved in preventing silencing of self-genes. Dr. Mello presented a hypothesis that these pathways...involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance ... to replace the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics...

  21. Lentiviral Prep Service

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    Collection
    ...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded...backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 ...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from...backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting...

  22. Worm Expression Resources

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    Collection
    ...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection CRISPR/Cas9 in C. elegans External...Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos... used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in...

  23. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing...Corn’s lab describe how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein...tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 genomes, and...template to increase efficiency of insertion and gene replacement. Care to share your favorite CRISPR ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing studies. These...desired edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list...

  24. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...the CRISPR-Cas9 system. Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving...CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned...versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...That so many scientists are tackling this type of genetic engineering is a testament to how amazing the downstream...

  25. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... your gene of interest. For ideal use, this gRNA should be cloned into a vector containing Cas9 and the...et al. (2016).  “MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.” Nat ...harnessed MMEJ to create a new method for CRISPR gene knock-in, termed PITCh (Precise Integration into Target ...than that observed with NHEJ. PITCh: Using MMEJ for gene knock-in Building on the lab’s previous work, Sakuma...plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA. The...Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step 4: ... is if the GFP-Puro will alter expression of the gene it follows. For increased versatility, it would ...

  26. Hot Plasmids February 2024

    Type
    Blog Post
    ...-invasive systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids previously...Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific ...peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing...lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due ...multiplexed guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization...Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter...Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain....

  27. Plant Plasmids and Resources

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    Collection
    ...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre... a new window) - Tools for gene and protein expression and mapping of gene expression in plants. Figshare...MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...plasmids containing promoters that drive general and species-specific gene expression (see the column ‘promoter...vectors: A modular plasmid assembly kit for multigene expression, gene silencing, and silencing rescue in plants...

  28. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ...Selectable Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

  29. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...reporter for rapid quantification of base-editing and Cas9 cleavage activity Article contributed by Angela ...lentiviral vector system that monitors both APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently...Harris lab showed that human APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more ... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where...function is more specific and much faster than any genetic or pharmacological manipulations on cells, and ...

  30. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a ...dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression... ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ... plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication...
Showing: 121 - 150 of 311 results