We narrowed to 210 results for: cycs

1. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   ...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still ...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

3. 6 Tips for Grant Writing

   Type

   Blog Post

   ... to improve your application for the next grant cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh...

4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   ...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

5. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

6. Academic vs. Industry Postdocs

   Type

   Blog Post

   ...industry postdoc will free you of the never-ending cycle of grant-writing that is academic life. Of course...

7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   ...stop emerging at night. An incubator with a light cycle makes for very predictable work! When flies first...

8. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ... different templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing...

9. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   ...work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

11. Plasmids 101: Colony PCR

    Type

    Blog Post

    ...dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA...

12. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    ...researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a minigenome...

13. Ras Pathway

    Type

    Collection

    ...exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4 CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2...

14. Hot Plasmids - August 2020

    Type

    Blog Post

    ...dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids...

15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ...Improved Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62...

16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    ...hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get ...

17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ... in the 80s and 90s, Joe was that kid who used recycled notebook paper, despite the fact that it was gray...

18. Bioinformatics at Addgene

    Type

    Blog Post

    ...Now, we could iterate our development and testing cycle faster due to the ease of jumping back and forth...

19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    ... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

20. Hot Plasmids: Fall 2024

    Type

    Blog Post

    ...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

21. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...while aptamers are more stable and can go through cycles of denaturation/renaturation. In vivo assays ...

22. How to Write a Scientific Review Article

    Type

    Blog Post

    ...is probably “a normal day.” The way I broke this cycle was to just start writing. A word of advice: when...

23. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination...

24. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...constant temperature, eliminating the need for thermocyclers. This is followed by in vitro transcription ...

25. March for Science

    Type

    Blog Post

    ...scientists then revise their hypotheses and the cycle begins again. No part of this process is inherently...

26. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...observed no negative effects on cell viability, cell cycle progression, or apoptosis with Cas9/gRNA expression...

27. Deisseroth INTRSECT Collection

    Type

    Collection

    ...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. ...

28. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    ...mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between...

29. Hot Plasmids: Spring 2025

    Type

    Blog Post

    ...identification of general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-...

30. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...to subject your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5...