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We narrowed to 235 results for: crispr grnas

Showing: 161 - 200 of 235 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...techniques such as CRISPR are easier to use and are more efficient at inactivating genes. CRISPR can target a...have developed new CRISPR-based methods for various knock-ins, discussed in our CRISPR 101 eBook. It’s ...genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems ...systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there are no suitable PAM sequences...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ... to create conditional floxed alleles. Although CRISPR is very good at making knockouts, knocking in large...thirty years ago. As traditional knockout and new CRISPR tools continue to be perfected, the production ...

  2. Plant Plasmids and Resources

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    ... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology... The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, ...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice...technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants...truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species...

  3. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ... out our CRISPR Featured Topic Page Learn about RNA Targeting with Cas13a Learn about CRISPR DNA Base ...Resources on Addgene.org Browse All CRISPR Plasmids Check out our CRISPR Guide Pages ...enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified...C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing...programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two years after that, the...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine... targeting work with Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The...

  4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ...editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series Resources...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the... Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month...Addgene.org Find base editors for your research Read our CRISPR guide Find plasmids for your research ...

  5. dTAG - You're it!

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    Blog Post
    ...Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs ...Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional Mouse Models CRISPR 101: Cas9 Nickase Design... selective degradation of FKBP12F36V-BRD4 using CRISPR/Cas9-mediated knock-in, and to evaluate in vivo...ENL-FKBP12F36V and endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7 and...-MELK and endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7, ...were engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated knock-in. Featured molecule: dTAG...

  6. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ... Request NgAgo Find CRISPR Resources Catch Up on Your CRISPR Background ...genome engineer sharing the stage with much-lauded CRISPR-Cas9 is DNA-guided endonuclease NgAgo! We'll discuss...development before it can truly be pitted against CRISPR head-to-head.  Exciting features of NgAgo First...which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and NgAgo genome engineering methods have ...Yourself On the other hand, the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...had tested and compared NgAgo head-to-head with CRISPR-Cas9. The survey had two sections, the first section...

  7. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience, including transgenic...neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant...). Created with BioRender.com. Before using gRNAs in vivo, they should be screened for activity in...molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for ....     References Liu, Yanxia, et al. "CRISPR activation screens systematically identify factors that drive...

  8. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ...Addgene.org Catch up on your CRISPR background with our CRISPR Guide Browse CRISPR plasmids for base editing...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ... indels were observed than point modifications. CRISPR base editing persists through multiple cell divisions...editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine...which you can learn more about in this blog post: CRISPR 101: RNA Editing with Cas13. Finally, the development...adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing. Nature Biotechnology...

  9. Validated gRNA Sequences

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...Addgene's CRISPR Plasmids and Resources page have been used to indicate the Cas9 application the gRNA was designed...details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table...

  11. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated...Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...but we do distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Curr Protoc Mol Biol. 2016 Jul 1;115..., Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  12. Lentiviral Prep Service

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    ...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...libraries 92385 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...Root 92386 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting...genes. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9...73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting...Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control...

  13. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for...base. If you heard about CRISPR, one question probably comes to mind: can CRISPR be applied to fix the defective..., Y. Multiplex CRISPR-mediated base editing in rice. In Genome Engineering: The CRISPR-Cas Revolution,...Addgene blog Read all of our CRISPR 101 blog posts Download the CRISPR 101 eBook Read our blog posts ... to facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types of repairs...et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with...adenine (A) to a guanine (G)? You may wonder if a CRISPR-base editor can perform this change. However, there...

  14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...technologies like CRISPR. Figure 1: An overview of the general schematic of CRISPR/Cas based COVID...check out this table.  All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive and...Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide Browse our entire COVID-19 plasmid...detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity:10-100 copies of...Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic...the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as ... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...deacetylases. CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made .... For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR Epigenetics resource. Transcriptional...potential advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors... on Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of...modification at specific genomic loci is possible using CRISPR, and Addgene has a number of tools for this purpose...targeted to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused...double strand breaks. Below you'll find some of the CRISPR-based epigenetic modifiers available from Addgene...

  16. Sequencing Options for CRISPR Genotyping

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    Blog Post
    ...View Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus...steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR genome editing...discussed in CRISPR 101: Validating Your Genome Edit, there are a variety of options for CRISPR genotyping...Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional Resources on ...target gene. The validation process, also known as CRISPR genotyping, is critical to demonstrating causal...biology. It is key to address these concerns as CRISPR use grows across the life sciences and to establish...moves toward standardized thresholds for validating CRISPR experiments, many are turning to next-generation...

  17. Pushing the Limits of DNA Assembly

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    Blog Post
    ...Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...of base pairs. Cloning sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be laborious... plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is...constructs with multiple point mutations Cloning sgRNAs Production of Yeast Expression Cassettes Generating...synthetic oligo to generate a single-guide RNA (sgRNA). Using only one single-stranded oligo, instead ...to create a double-stranded oligo. Generating an sgRNA construct becomes as simple as combining your favorite...

  18. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ... Encodes gRNA/shRNA A plasmid with one or more gRNA or shRNA sequences cloned into it. gRNAs are used...used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are used to knockdown expression...gene contained within your insert (or targeted by a gRNA/shRNA) and select the species that the gene or insert...

  19. Typing CRISPR Systems

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    Blog Post
    ...blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: RNA Editing with Cas13 Build your CRISPR vocabulary...Additional resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references ...Classification Structure Principles of CRISPR classification While CRISPR systems now seem neatly organized...versus class 2 There are two CRISPR classes: Class 1 and Class 2. Class 1 CRISPR systems use multi-Cas effectors... Type I CRISPR systems make up the majority of Class 1, and are the most common type of CRISPR overall...complex of the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type ...immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo...

  20. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post
    ...Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org...

  21. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post
    ...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector. When followed...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...

  22. Bikard Lab - CRISPR Repression Collection

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    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur...a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently... DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either...

  24. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease...Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use Cas9...intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field...delivery Epitope tagging of mammalian genes using CRISPR depends on successful delivery of three reagents...using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA...tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were pre-assembled...csRNP knock-in experiments If your lab is new to CRISPR/Cas9 experiments, there is no need to worry at ...

  25. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand... the most intuitive CRISPR MoClo application is to assemble multiple unique sgRNAs into a multi-TU targeting...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...edit optimization, and more. While there are many CRISPR-Cas libraries and tools available for certain model...biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both monocots...MoClo can help you achieve it! More recently, a CRISPR MoClo kit termed Fragmid was developed for mammalian...

  26. Plasmid Collections

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    ...pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  27. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post
    ...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How...Educational Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide...primers to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the ...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  28. Worm Expression Resources

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    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and...

  29. Microbiology Resources

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    ...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...Check out our CRISPR reference page for CRISPR resources, such as depositor protocols and gRNA design software...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression... specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology : Browse our deposited...editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering blog post...

  31. Zhang Lab CRISPR Page

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    ...CRISPR Cas9 gRNA plasmids from the Zhang lab... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...

  32. Bacterial Expression Systems

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    ... list of our bacterial CRISPR plasmids, see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of...Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive...list of our bacterial CRISPR plasmids, see here . pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...alter bacterial genomes. From recombineering to CRISPR , this collection contains a variety of tools to... of customizable guide RNA (gRNA) for bacterial gene disruption or knockdown. For a more comprehensive...comprehensive list of our bacterial CRISPR plasmids, see here . pGRG Series Various Plasmids Tn7 Transposition Nancy...

  33. Zebrafish Plasmid Collection

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    ...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology... zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones....examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...

  34. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post
    ... about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here ...genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been shown to outperform other popular CRISPR libraries...repressor (CRISPRi) or an activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries...advantages of the optimized human CRISPR libraries. Brunello: human CRISPRko sgRNA library The Brunello library...analysis of the sgRNAs, Brunello even with only a single sgRNA outperformed another CRISPR library with ...with 6 sgRNAS, highlighting the benefit of sgRNA design in CRISPR libraries. Thus, the Brunello library proves...

  35. Lentivirus Plasmids

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    ...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...

  36. Hot Plasmids: Spring 2025

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    ...editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the pithy name "site-specific...site-specific target-primed insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...

  37. Allen Institute for Cell Science Plasmid Collection

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    ... Plasmid Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide...including: data and images from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell ...

  38. Neurodegeneration Research Collection

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    ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...expressing wild type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative disorders..... Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al....

  39. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    Blog Post
    ...screening using CRISPR Find all of our CRISPR pooled library blog posts Check out all of our CRISPR blog posts... of CRISPR screen data that might be able to help you out. What is the Open Repository of CRISPR Screens... accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated...the Open Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell ...   Include your CRISPR screen data in ORCS  Researchers who wish to have their CRISPR screen data included...Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your... post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that your...

  40. Rett Syndrome

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    ...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources...'s Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new...below: Base Editing RNA Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch...
Showing: 161 - 200 of 235 results