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We narrowed to 319 results for: virus

Showing: 201 - 250 of 319 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...ready-to-use AAV preparations are labeled with the green virus particle on our biosensors page. Whether you’re...

  3. To Each HIS Own

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    Blog Post
    ...histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks...

  7. Caltech Systemic Capsids

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    Collection
    ...tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Text...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... of insect cell expression systems include that virus production can be quite time consuming and that ...expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems are.... coli or yeast cells. The only problem with baculovirus systems is that the infected host cell is eventually...

  9. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...helper plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming ...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that...outbreaks of food poisoning, the difference is virulence plasmids! This category includes genes that help...instance, vectors derived from the Agrobacterium Ti virulence plasmid are now used routinely in biotechnology...

  10. Hot Plasmids - October 2020

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    Blog Post
    ...Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both...improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies...vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector with N-terminal GST pGCS-N5...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome Modifications...

  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... consider including more background on how your virus of interest causes cancer, as well as therapeutic.... She studies the interaction between Human Papillomavirus and the innate immune system, and is interested...

  14. Hot Plasmids February 2024

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    Blog Post
    ...lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive ...

  16. Common Injection Routes in Mice

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    Blog Post
    ...including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic...

  17. CRISPR Plasmids - Prime Edit

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    Collection
    ...other prime editors use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the...

  18. AAVED

    Type
    Collection
    ...for openly shared communication: Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

  19. Chemogenetics AAV Preps

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    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  20. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...relatively laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  23. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector for gene therapy of human diseases... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral...the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV does not integrate into...

  25. Plasmids 101: Protein tags

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    Blog Post
    .... One of the best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is ...

  26. AAV Packaged on Request

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    Collection
    ... Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...

  30. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  32. p53 Pathway

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    Collection
    ...to mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere ...

  33. Evolution of Lab Techniques

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    Blog Post
    ...and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...

  36. Control AAV Preps

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    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  40. Tetracycline Inducible Expression

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    Collection
    ...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR portion...

  44. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    ...potential to overcome some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...(non-LTR TEs). LTR retrotransposons are like retroviruses both in their structure and mechanism of replication... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...

  45. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  49. Optogenetics AAV Preps

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    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  50. Retrograde AAV viral preps

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    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...
Showing: 201 - 250 of 319 results