We narrowed to 395 results for: ICL

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

...10 Hot Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids...access these snippets here or from their respective articles on the Hot Plasmid webpage. We hope you enjoy ...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

...capabilities but are small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with CRISPR...

...Resources , links to collections of open-access articles, protocols, and other COVID-19 resource collections...

... lab experiences, findings, and experiments as articles written for general audiences. So far, we are ...feedbacks from our non-scientist readers about how our articles have helped them understand different topics of...launch an e-magazine this year which would feature articles, posts, and sci-art that would ultimately give...Addictive Brain. A contribution can either be an article, a short post, a video, or a piece of sci-art.  ...

...lab used SNAP-tag to decorate synthetic viral particles with BG-linked antibodies to drive cell type-specific...intracellular targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778  Erdmann...A flexible chemical tag. Nature Methods, 2(4), Article 4. https://doi.org/10.1038/nmeth749  Strebinger...envelope design. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-40788-8  ...capping protein. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-39655-3  ...

...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Minimum titers...

...of AAV2 this is thought to be due to the viral particle’s affinity for heparan sulfate proteoglycans on... into the cell culture supernatant, the viral particles associate with the cell membrane and are taken... we aimed to prevent the uptake of AAV2 viral particles by our packaging cells by treating the cells with...fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference between untreated...

... examples of quality control data. Help Center Articles Additional details about viral vector production...

...appropriate factors displayed on the outside of the particle to interact with the host. This is the role of...order. This will all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA...capsid protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked...proteins required for packaging of the virus into particles. E2A/E2B are responsible for viral replication...

...acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene...

... and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution of the protein...monkey neurons, the large majority of the gold particles were in the cytoplasm of dendrites, and only a...and green arrows point, respectively, to gold particles localized in the cytoplasm or bound to the plasma... protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: ...

...of new particles. With subsequent cell divisions, the fraction of cells containing the particle exponentially...assembly of fluorescent proteins into one bright particle that segregates into one cell during cell division...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent...

...expression or localization in cells, or even single-particle tracking of the bound complex. There are so many...EBFP2(1–10) can be used with GFP(11)). Some of the articles linked below only provide one fragment of the ...Protein Tags Browse the table below, and click the Article link to find all the plasmids from the associated...associated paper:   Color Base FP Description Article (Link to Plasmids) PI Blue EBFP2 EBFP2(1–10...

... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...Nervous about handling viruses? That’s okay! In this article we will discuss general viral safety considerations...you’ll need to take extra steps to ensure that particles are not sprayed into the air. For lentivirus and...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...