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We narrowed to 396 results for: ICL

Showing: 81 - 100 of 396 results

  1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...of AAV2 this is thought to be due to the viral particle’s affinity for heparan sulfate proteoglycans on... into the cell culture supernatant, the viral particles associate with the cell membrane and are taken... we aimed to prevent the uptake of AAV2 viral particles by our packaging cells by treating the cells with...fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference between untreated...

  2. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...appropriate factors displayed on the outside of the particle to interact with the host. This is the role of...order. This will all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA...capsid protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked...proteins required for packaging of the virus into particles. E2A/E2B are responsible for viral replication...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...of new particles. With subsequent cell divisions, the fraction of cells containing the particle exponentially...assembly of fluorescent proteins into one bright particle that segregates into one cell during cell division...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent...

  4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ... and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution of the protein...monkey neurons, the large majority of the gold particles were in the cytoplasm of dendrites, and only a...and green arrows point, respectively, to gold particles localized in the cytoplasm or bound to the plasma... protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: ...

  5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...expression or localization in cells, or even single-particle tracking of the bound complex. There are so many...EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide one fragment of the ...Protein Tags Browse the table below, and click the Article link to find all the plasmids from the associated...associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for ...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...Nervous about handling viruses? That’s okay! In this article we will discuss general viral safety considerations...you’ll need to take extra steps to ensure that particles are not sprayed into the air. For lentivirus and...

  7. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...

  10. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

  11. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  12. Synthetic Biology - Algal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  13. Synthetic Biology - Bacterial

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  14. Synthetic Biology - Fungal

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  15. Synthetic Biology - Mammalian

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  16. Synthetic Biology - Plant

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  17. Synthetic Biology - Worm

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  18. Synthetic Biology - Yeast

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...

  19. Synthetic Biology - Strains

    Type
    Collection
    ...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...
Showing: 81 - 100 of 396 results