...review articles, such as: Sander JD & Joung JK, Nature Biotech, 2 March 2014. Or browse the articles related...Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options for CRISPR plasmid...answers on the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...

...of AAV2 this is thought to be due to the viral particle’s affinity for heparan sulfate proteoglycans on... into the cell culture supernatant, the viral particles associate with the cell membrane and are taken... we aimed to prevent the uptake of AAV2 viral particles by our packaging cells by treating the cells with...fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference between untreated...

...appropriate factors displayed on the outside of the particle to interact with the host. This is the role of...order. This will all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA...capsid protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked...proteins required for packaging of the virus into particles. E2A/E2B are responsible for viral replication...

...of new particles. With subsequent cell divisions, the fraction of cells containing the particle exponentially...assembly of fluorescent proteins into one bright particle that segregates into one cell during cell division...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent...

... and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution of the protein...monkey neurons, the large majority of the gold particles were in the cytoplasm of dendrites, and only a...and green arrows point, respectively, to gold particles localized in the cytoplasm or bound to the plasma... protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: ...

... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

...expression or localization in cells, or even single-particle tracking of the bound complex. There are so many...EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide one fragment of the ...Protein Tags Browse the table below, and click the Article link to find all the plasmids from the associated...associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for ...

...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...Nervous about handling viruses? That’s okay! In this article we will discuss general viral safety considerations...you’ll need to take extra steps to ensure that particles are not sprayed into the air. For lentivirus and...

...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find...

...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here’s what you’ll find in this article: CRISPR-based editing in organoid Biosensors for...

...of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter so be... expression. The plasmids associated with this article can be obtained through Addgene: Rapamycin-inducible...course of gene activation using this system in the article cited below. Scientists can obtain dCas9-trCIB1...trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene. Nihongaki et al., Chemistry and...TOP2 constructs; see Supplementary Note 3 in the article for more info) are available. Furthermore, there...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Here's what you'll find in this article: An improved cell-free system to examine biosynthetic...

...the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. ...100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes...

...types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

...more viral particles. Eventually the cell lyses releasing the newly formed viral particles that can infect...are packaged into replication-incompetent phage particles with assistance from a ‘helper’ phage prior to...

...a brief overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes...different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios, making...and may be a form of transcytosis where the AAV particle is taken up on one side of the cell and released...cell. While there is always a risk that viral particles will diffuse long distances within the extracellular...