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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 969 results for: LAT

Showing: 81 - 100 of 969 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ... these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  2. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed... to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...binding site (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 - introduction of five...

  4. Worm Expression Resources

    Type
    Collection
    ...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...nematodes, including C. elegans and C. briggsae. Other related miniMos plasmids can be found on the Jorgensen ...genetics, genomics and biology of C. elegans and related nematodes. WormBook - A comprehensive, open-access...original, peer-reviewed chapters covering topics related to the biology of C. elegans and other nematodes...

  5. Viral Vectors

    Type
    Collection
    ...vector plasmids available at Addgene and other related resources. ...transfer in clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity... are typically used to encode, propagate, and manipulate genetic information, viruses are a powerful tool...These engineered viral genomes are also modified (relative to natural viruses) for safety through the removal...

  6. NETRF

    Type
    Collection
    ...effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...CRISPR plasmids for activating or upregulating gene expression in various model organisms....Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ... to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid...

  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ...normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although cancers...states. Ras Ras is a GTPase that is essential for regulating the growth of the cell. p53 p53, the most frequently...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...the spread of infection throughout the entire population. Type VI enzymes that function in mammalian cells...mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information...

  10. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  11. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  12. CRISPR Plasmids and Resources

    Type
    Collection
    ...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ...insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator peptide ...Purify dCas9 fused to an epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence...

  13. Retrovirus Plasmids

    Type
    Collection
    ...called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber...

  14. Antibody Production

    Type
    Collection
    ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

  16. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...

  17. CRISPR References and Information

    Type
    Collection
    ...and correspond to edited and non-edited cell populations. For more information, see the Hsiau et al. (.... protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR knock-in design at scale. It uses an...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...

  18. Synthetic Biology - Strains

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search the table...

  19. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing...

  20. Synthetic Biology - Metabolism

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include...
Showing: 81 - 100 of 969 results