We narrowed to 1,015 results for: NTS

...cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily... cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains...

...Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...

...imaging in deep-tissue organs or whole animals presents unique challenges. Genetically-encoded fluorescent...photoreceptors. Spectrally distinct fluorescent iRFP variants have been shown to have high brightness and allow...

... , which also includes other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

...attributed in part to the difficulties in getting all components of the Cascade complex to express equally. To...though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism...

...CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with...

...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs.... ligands, and how to use these tools in your experiments, please see our Chemogenetics Guide . Chemogenetics...

...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...available. To find plasmids containing other RNAi components, like argonaute, please search our full site ...

...part of an integrated pipeline, but individual components can be used separately for additional applications...listed in Park & Sheridan et al., 2025). These reagents have been used in expansion microscopy workflows...

...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors,...

...receptors and ligands (Glypicans and Integrins), components in the synapse (Neurexins and Neuroligins), and... to identify and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate...

...’s recommended applications. The specific QC experiments performed varies for each antibody catalog item... mammalian cells. To learn which specific QC experiments were performed on your lot, please email help...

... by the Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a mutant...68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD plasmid...identifies a staggering ~250,000 phosphorylation events in the proteomes of higher mammals (2). How can... the importance of any of these phosphorylation events on the activity of a given protein? Classical methods...5). Optimization of this system defined three components required for translation (Figure 1): A tRNA ...the pSer-incorporated polypeptide. These three components were shown to effectively incorporate phosphoserine...inactive without requisite dual phosphorylation events on its activation loop, but can be made constitutively...

...efficiencies of multiplex editing at each site. A 75-nt human cysteine tRNA stood out from their initial ...array, DAP arrays use tRNA itself, as short as 75-nt, to both drive and process the tRNA-gRNA arrays, ...both efficiency and multiplexity, with these constraints? The Gao lab first thought to enable MBE by ...the DAP array, the Gao Lab packaged MBE and MPE elements for deliveries via adeno-associated virus (AAV...

...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...

...attractive for imaging and fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds...plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab and described in...

...fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction and inhibition...inhibition in real time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link ...

... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Gate cloning method to assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using...

...cis plasmid) containing the transgene or genetic elements of interest to introduce in cells. A packaging...AAV vector guide for more information about AAV components, generations, viral production, and viral safety...