We narrowed to 229 results for: PID
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TypeCollection... and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression cassettes, which...transcript Bullock pCRISPathBrick Bacteria Allows rapid modular assembly of natural type II-A CRISPR arrays...
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Fluorescence Titering Assay
TypeProtocol...associated with freeze-thaw. If using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use... -
General Transfection
TypeProtocol...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...are reflecting on how to help scientists in this rapidly changing environment. In particular, we are working... -
Lentivirus Production
TypeProtocol...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post.... TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger nucleases to transcription... -
Reflections and Looking Ahead
TypeBlog Post...are shared. The scientific landscape is evolving rapidly, and with it, the needs of researchers worldwide... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...score as hits in pooled screens, facilitating more rapid follow-up. We hope the biological community will... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...identify those that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said... -
Transfection for Recombinant Antibodies
TypeProtocol...have dissolved. Note: The powder should dissolve rapidly (with 20 min) but check for the presence of particles... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant, Drosophila... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...S A. 2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering ... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post... Grants that support us in our mission. While rapidly expanding our COVID-19 resources has been our focus... -
What's New in CRISPR - March 2020
TypeBlog Post...genome-wide off-target DNA editing. Using several rapid and cost-effective screening methods, David Liu’... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol... to collect the next fraction. The flow will be rapid at first and will progressively slow down. Option... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...elongate the new target DNA strand accurately and rapidly. Repeat steps 2-4 25-30 times. Final Extension ... -
Fostering communication in remote collaborations
TypeBlog Post...various tools, this may happen over email or using rapid communication tools such as Slack. Share data and... -
Hot Plasmids - October 2022
TypeBlog Post...HcKCR2 have high selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use ... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post[email protected] to submit their published data for rapid curation review and upload. In addition to our main...