We narrowed to 749 results for: REV

...-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs...33277466 (Link opens in a new window) Content last reviewed on 26 September 2025. Do you have suggestions ...

...understood, and there are no effective therapies to prevent, treat, or cure most of these disorders. An integrated...age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists mainly of...

...bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish,...available for human and mice PDF, 276 KB Content last reviewed on 9 June 2025....

...out genes or introducing specific mutations can reveal important insights into gene function. These genetic... CRISPR-Transposons Return to top Content last reviewed on 17 June 2025. Do you have suggestions for other...

...Sox2 Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cell. 2015... Lentiviral Human Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from...

...pBAD-PSmOrange2 - Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein...proteins created by Talley Lambert Content last reviewed: 25 September 2025 Do you have suggestions for...

...submit it to a journal that uses a peer review system. Peer review in traditional journals happens before...blind (meaning you don’t know who the reviewers are). If the reviewers and the editor decide that your manuscript...several rounds of revision before acceptance. F1000Research uses a novel form of peer review that occurs post-publication...referee reports. If the reviewers suggest that the manuscript requires revisions, the authors can publish...is a relatively new development, and it allows reviewers and other researchers to access the data and make...and reliability of science publications. Peer reviewing To make sure your manuscript is perceived as a...manuscript needs to be revised before it can be published, this can delay publication and manuscripts ...

...them! PE2: Improving reverse transcriptase efficiency Building upon prior reverse transcriptase research...humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases...prime editor consists of a Cas9 nickase fused to a reverse transcriptase. The Cas9 nickase is guided to the...sequence hybridizes to the target site, and the reverse transcriptase domain copies the desired edit, directly...wild-type Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase...every ~8 base pairs on either DNA strand, many previously developed base editors with a <8 base-pair editing...the edit, the Liu lab turned to a strategy they previously used when they developed base editing. By nicking...

...systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir (small...and exogenously expressed proteins. Here we will review what advantages degron tags can offer over other...systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can...the drug has been removed. The degradation and reversal are rapid compared to other systems because the...comes to degrons, you have options! Below we will review the 4 most common types of degrons and some of ...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...addition to having the coolest name of all the tags reviewed here, the SMASh tag does offer a few other unique...

...formal peer review. Some organizations, including some journals, also support open peer review or curation...journal-based peer review can be prone to biases or errors, with a lack of transparency in most review processes...authors on a public server independent from peer-reviewed publishing in a journal.  Accelerating scientific...scientists and advocates have argued that while peer review is valuable, modern scientific publishing has a...or endorsements. In contrast, journal-based peer review is usually a confidential process among a few individuals...productivity that can be shared with hiring and review committees, as opposed to the opaque (and, admittedly...preprints, and a growing number of funding bodies and review committees encourage and consider preprints as ...

...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...of a nitrogen source. S. cerevisiae  no no   LEU2 L-leucine no S. cerevisiae yes - This can complement...explain how they differ from the bacterial elements previously described. Why do scientists use yeast vectors...pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features required...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation ...if specific regulatable elements are included (reviewed here). Plasmids for use in S. pombe, on the other... TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1 alters some yeast phenotypes. This...

...publication of peer reviews so we can see how and why papers change throughout the review process. This will...reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers should be able...incredibly useful technique simply by following the abbreviated methods section found within a published manuscript...additionally averse to producing papers that repeat previously published results. This over-reliance on the ...will hopefully push reviewers towards more productive criticisms that focus less on perceived novelty ...recognizing that we’re biased and taking steps to prevent our biases and goals from effecting our experiments...things I’ve read comes to this: we need others to review our experimental designs and we need to be transparent...

...and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene...are there even other yeasts? In this blog we will review an alternative model yeast strain and discuss why...used yeast strains: S. pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model ...genetic manipulation The first eukaryote (S. cerevisiae) to have its genome fully sequenced There are...wondering if the “biotech yeast” is S. pombe or S. cerevisiae. Actually, it’s neither. The most commonly used...phaffii and other yeast strains.     S. cerevisiae S. pombe K. phaffii Growth properties... is only distantly related to S. pombe and S. cerevisiae; evolutionarily it evolved much slower and bears...

...only active under specific circumstances. In a previous post, we discussed inducible promoters, which ...bind to the operator. The bound repressor then prevents transcription from occurring, which means that...to the tetracycline operator sequences (TetO), preventing transcription. In the presence of tetracycline...binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...as well as the inducible manner detailed in our previous post. In the past 30 years since the original ...binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...as well as the inducible manner detailed in our previous post. While originally developed for use in mammalian...

.../10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav...sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration Collection..., 2019, including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of host cell ...traditional AAVs, the Becirovic Lab has developed REVeRT, for gene delivery using reconstitution via mRNA...2023 under a CC-BY license.  The authors used REVeRT to functionally reconstituting coding sequences...disease-relevant large proteins. They demonstrated REVeRT in various contexts in vitro and in vivo, including...human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous genetic material...

...-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9... cleave the lentiviral RNA, preventing packaging of the virus.  Reversing the orientation of the direct...RNA from Cpf1-mediated cleavage. This reversing would also prevent processing of the crRNA array when expressed...generally more efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct repeats when...expressed in cells, but notice how Zetsche et al reverses the orientation of the promoter driving expression...(bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression of...

...Performing peer review at the request of your supervisor is not considered peer review. The peer review request...achievements. Participating in the scientific review process as a reviewer as proof of your judging the work of...already reviewing papers that you should start contacting journal editors to request to be a reviewer. Proactivity... Participating as a judge (including as a peer reviewer for journals, acting as a journal editor) of the...you specifically. You must also complete the peer review for it to be counted as proof of judging. Merely...case.  You can still assist your PI in the peer review process, but remember you cannot use it as documentary...evidence for your case – the invitation to be a peer reviewer needs to be addressed to your own personal email...

... useful. Step 4: Read and learn from the reviewers Reviewers may take 6-12 months to give feedback on...of the art technologies. Therefore, take time to review the literature and offer educated insight into ... the agency and benefits the field reminds the reviewer why your project is the project to fund. Using...limits) provided in this section will convince the reviewers that the proposal is strong, well-thought out,...your advisor to supply a budget form used in a previously funded grant application to see how funds were...application. Make sure to invest time and consider the reviewer's comments. Whether the proposal is funded or not...for improvement, deliver outsider opinions, and reveal items to include for the next application. Although...

...was to be revisited at meetings when sufficient time, additional public fora for data review, and more... Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist working... were born in a twin live birth several weeks previously. While this claim has yet to be independently...report on the ethics of “Novel techniques for the prevention of mitochondrial DNA disorders.” This document...public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The...Council began their research into both novel and previously established ethical issues raised around mitochondrial...stop shuffling our feet on necessary actions to review and decide on the future of genome editing technology...

...guide always gets an expert review, separate from the author, and a second review from someone less familiar...channel just for asking people if they can write or review things and another for asking specific science ...answers from our incredible scientists. Expert reviews on blog posts are done on a case-by-case basis,...case of the antibody guide, primary research and previously written Antibody 101 blog posts were used, as...panel recommendations. A few discussions in the review process led to quick dives back into the literature...sharing repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making...content is an iterative process. We are constantly reviewing the resources we currently offer to identify gaps...