...accessible to practically everyone.     Fig. 1: Open source hardware builds   Since I started...components. In this field, often referred to as 'frugal science', existing components are adapted and combined...

...available as a kit from Addgene.         References 1. Chen, Baohui, et al. “Dynamic Imaging of Genomic ...b. when a gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system... components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase...

...lentiviral transfer plasmids are based on the HIV-1 virus. For safety reasons, transfer plasmids are all...shRNA‐expressing lentiviral plasmids, such as pLKO.1, use a U6 or H1 promoter in order to drive RNA pol...protocol for using the popular cloning vector pLKO.1. Trono Lab Resources: Lentivectors Toolbox and Maps...selection. Desfarges S, Ciuffi A. Viruses. 2010. Jan;2(1):111-30. PubMed . Altering the tropism of lentiviral...Curr Gene Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA...cells by a lentiviral vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, and Trono...

...algae Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific...based on the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester and release proteins...concept of optogenetics. 2012 Prog Brain Res. 196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan...diversifying and extending optogenetics. Cell. 196:1-28. PMID 20303157 Han X, Boyden ES. 2007 Multiple-...Limitations and Future Developments. Exp Physiol. 96(1): 19–25. PMID 20621963 Mattis J, Tye KM, Ferenczi ...both the identification of novel ChRs from other algal species and the development of synthetic variants...

...impacts of neural circuits on behavior. Figure 1: An overview of the chemogenetics system. Why use...Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent...

.... Figure 1. DREADDs, their ligands, and signaling properties. Please see the text or table 1 for details...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons...delivery, while PSEMs have an effect for only 0.5-1 hours after delivery. Chemogenetic Ligand. The chemogenetic...Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent...

...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have...tournament or beer hour (only if it’s cool with your legal department) right in your breakroom. 5. Throw some...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been...

... your experiment. Libraries may be available in a 1-plasmid system, in which Cas9 is included on the gRNA-containing...sequence for Staphylococcus aureus Cas9 (SaCas9) is ∼1 kb shorter than SpCas9, SaCas9 can be efficiently ...sequences containing a G nucleotide at position 20 (1 bp upstream of the PAM) may be more efficacious than...population. Some cells may be wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack...Giovannangeli C, Concordet JP. Nat Commun . Mar 19;9(1):1133. PMID: 29556040 Directed evolution of CRISPR-Cas9...Jung I, Kim D, Kim S, Kim JS. Nat Commun . Aug 6;9(1):3048. PMID: 30082838 Engineered anti-CRISPR proteins...DE, Piccioni F, Doench JG. Nat Commun . Dec 21;9(1):5416. PMID: 30575746 Synthetic CRISPR-Cas gene activators...

...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab... weird face your fears and unleash creativity. Gallery Books. Boyes, A. (2019, November 13). 5 mental ...

...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically...Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA (2019) CasX enzymes comprise a distinct...

...complex. So how exactly does this work? Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter ...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ...technique to beautifully demonstrate light-dependent Gal4 transcriptional activation and Cre recombination...

...mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination! Tissue culture media often have indicators... mycoplasmas but also avoid other bacterial and fungal contamination. Proper PPE Since humans are the ...

...human cytomegalovirus EF1a Constituitve Strong mammalian promoter from human elongation factor 1 alpha...expression UAS Specific Drosophila promoter containing Gal4 binding sites Bacterial Promoters Promoters in bacteria...