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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,023 results for: nts

Showing: 81 - 100 of 1023 results

  1. CRISPR References and Information

    Type
    Collection
    ... pipeline for the analysis of genome editing experiments. It enables rapid and intuitive interpretation... for the purpose of designing and evaluating experiments. Developed by the Hyongbum Kim Lab . Green Listed... range of reference genomes, including animals, plants, bacteria, fungi, protists, and viruses. Developed...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ...these ORF fragments (which have become exons) determines the logical expression requirements of Cre and...References The development of powerful molecular reagents for biology have propelled our understanding of... for the molecular design and testing of novel INTSRECT plasmids for groups interested in producing their...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs ...screened for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more...keep the following in mind: CRISPR screening experiments require electroporation to amplify the pooled...confirm library representation. CRISPR screening experiments require large quantities of cells and therefore...

  4. Antibodies

    Type
    Collection
    ...recombinant antibody collection. These affinity reagents are expressed and purified from plasmids in our...research. We are building a collection of affinity reagents that are expressed from plasmids. These antibodies...therefore makes the sequences of these affinity reagents available to scientists. Additionally, we have...

  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter...

  6. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA sequence compatible with? CRISPR knockout experiments use targeting sequences within exons, whereas...whereas CRISPR activation or repression experiments use targets within promoters. Do you have gRNAs you'd like...wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created...

  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...attributed in part to the difficulties in getting all components of the Cascade complex to express equally. To...though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism... expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication...

  8. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Viral Plasmids Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook...) are small viruses originally discovered as contaminants of adenovirus stocks. One major advantage of...our AAV Guide for more information about: AAV components Common uses of AAV Viral integration AAV serotypes...

  9. p53 Pathway

    Type
    Collection
    ... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget....2147/OTT.S53876. PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field...

  10. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... for use as donor and acceptor pairs in FRET experiments. The efficiency of the energy transfer is measured...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian...

  11. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... This technique creates diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert ... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...vector, and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA...CRISPR/Cas9 toolkit for multiplex genome editing in plants Plant Plasmids for six gRNA module vectors, including...High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple...

  13. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...more important because you have to target within ~30 nt of your proposed edit, which means there are so few...base editors, the intended edit must be in a 5 - 10 nt window relative to the PAM, and bystander edits are...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...HDR for introducing edits. The same locational constraints are even more exquisite for the so-called base... JG (2020) Design and analysis of CRISPR–Cas experiments. Nat Biotechnol 38:813–823 . https://doi.org/...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated in zebrafish...resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next generation...mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays (assembled...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...other mutants Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR...eNpHR and variants Jaws WiChR stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming the specificity of the ...activation of endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion ...technology for studying the function of genetic elements. To broadly enable the application of Cas9 in ...

  17. Validated gRNA Sequences

    Type
    Collection
    ...been experimentally validated for use in CRISPR experiments. This datatable is compiled from plasmids in ...gRNA sequence compatible with? CRISPR knockout experiments use targeting sequences within exons, whereas...whereas CRISPR activation or repression experiments use targets within promoters. When possible, the categories...

  18. Synthetic Biology - Plant

    Type
    Collection
    ...collection of synthetic biology plasmids for use in plants....collection of synthetic biology plasmids for use in plants. Plant Plasmids Search the table by keyword or ...

  19. TALEN Expression Vectors

    Type
    Collection
    ... TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors...This page lists plasmid vectors into which DNA fragments encoding TAL effector repeat arrays (assembled...

  20. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily... cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains...
Showing: 81 - 100 of 1023 results