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We narrowed to 322 results for: phy

Showing: 81 - 100 of 322 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...

  2. Antibody Guide

    Type
    Collection
    ...responsible for driving allergic reactions such as anaphylactic shock Monomer with a valency of 2 IgG The most...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light, oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui,...study multiple aspects of cellular activity and physiology. However, there are a number of factors that ...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ...contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University of Manitoba. ...comparisons to enhance our understanding of receptor physiology and the effects of metabotropic and ionotropic... Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at the University of Manitoba. ...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    .... Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 Nishimasu...from making off-target cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions...

  6. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

  7. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids, Blugene...their jaws into a leaf, and dies as its muscles atrophy. After the ant’s death, the fungal fruiting bodies...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... The technique successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...Methods paper last year. The key to their bacterial phytochome-derived proteins, iRFP670, iRFP682, iRFP702, ...fluorescent proteins engineered from a bacterial phytochrome. Piatkevich et al (Nat Commun. 2013 Jul 10;4:...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...all-optical electrophysiology A major challenge in the field of all-optical electrophysiology is designing...provided the field with an improved tool for electrophysiology, minus the use of electrodes. The Cohen lab...- a real boon to those trying to understand physiological interactions and functions of their POIs. ...

  12. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... distancing? Many people are now calling this “physical distancing” since it’s important to stay virtually...message for some people. We should recommend #PhysicalDistancing. Social interactions and #social support ...

  15. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... because of effects of pH on protein stability (Murphy et al., 2013). However, the pH changes in our solutions...the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning...

  17. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria, or...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

  18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...researchers did the following: Improve sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic...membrane trafficking and localization. Improved photophysical properties The improved variants of iGluSnFR3...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...(Richardson et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision...sequence replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation...

  20. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...of applications within chemistry, biology, and physics, in this blog we will focus on those which can ...monitor the fundamental cellular building blocks. Physiological factors Unlike the previously described sensors...
Showing: 81 - 100 of 322 results