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We narrowed to 327 results for: phy

Showing: 81 - 100 of 327 results
  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    .... Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 Nishimasu...from making off-target cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions...
  2. Genetic Code Expansion

    Type
    Collection
    ...biology by providing access to new chemical and physical properties for studying, manipulating, and evolving...
  3. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...
  4. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...
  5. Six Spooky Science Stories and Halloween at Addgene

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    Blog Post
    ...and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids, Blugene...their jaws into a leaf, and dies as its muscles atrophy. After the ant’s death, the fungal fruiting bodies...
  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... The technique successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...
  7. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...Methods paper last year. The key to their bacterial phytochome-derived proteins, iRFP670, iRFP682, iRFP702, ...fluorescent proteins engineered from a bacterial phytochrome. Piatkevich et al (Nat Commun. 2013 Jul 10;4:...
  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...all-optical electrophysiology A major challenge in the field of all-optical electrophysiology is designing...provided the field with an improved tool for electrophysiology, minus the use of electrodes. The Cohen lab...- a real boon to those trying to understand physiological interactions and functions of their POIs. ...
  9. Antibodies 101: Affinity Tags

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    Blog Post
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...
  10. 25 Work From Home Tips for Scientists

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    Blog Post
    ... distancing? Many people are now calling this “physical distancing” since it’s important to stay virtually...message for some people. We should recommend #PhysicalDistancing. Social interactions and #social support ...
  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...researchers did the following: Improve sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic...membrane trafficking and localization. Improved photophysical properties The improved variants of iGluSnFR3...
  12. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria, or...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...
  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... because of effects of pH on protein stability (Murphy et al., 2013). However, the pH changes in our solutions...the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning...
  14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...(Richardson et al 2016) revealed some of the biophysical parameters that can influence the HDR/NHEJ decision...sequence replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation...
  15. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...of applications within chemistry, biology, and physics, in this blog we will focus on those which can ...monitor the fundamental cellular building blocks. Physiological factors Unlike the previously described sensors...
  16. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...biology at Virginia Tech, and earned a Ph.D. in Biophysics from UC Davis in 2007. Nikolai trained as a post-doc...ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute ...
Showing: 81 - 100 of 327 results