We narrowed to 962 results for: tes
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TypeBlog Post...from 40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...The SPARC system uses competitive recombination sites to selectively remove a stop cassette upstream of...expressed. By truncating one of the recombination sites in the construct to different lengths, the density... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...
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Neuroscience
TypeCollection... represent the official views of the National Institutes of Health.... -
Synthetic Biology - Networks and Gene Regulation
TypeCollection...and terminators repressors and activators logic gates Networks and Gene Regulation Plasmids Search the... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence... -
Antibodies
TypeCollection... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate... -
Individual REAL TALE Repeat Plasmids
TypeCollection...single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These 28 plasmids... -
CRISPR Plasmids - RNA Editing
TypeCollection...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine... -
Zinc Finger Consortium: Nuclease Expression Vectors
TypeCollection...in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains... -
Cancer Research Plasmids and Resources
TypeCollection...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...requires delivery of hundreds of gRNAs with target sites throughout the chromosome. Mammalian ID Plasmid ... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection... transcription factor and other protein binding sites. Browse, sort, or search the tables below for CRISPR... -
CRISPR Plasmids - Zebrafish
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... -
Structural Genomics Consortium Plasmids
TypeCollection...plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry and sfgfp... -
Botman-Teusink Yeast FP Collection
TypeCollection... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces... -
Mammalian RNAi Tools
TypeCollection...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows... -
CRISPR Plasmids - C. elegans
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...shared experience for the research community and facilitates scientific collaborations between institutions... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, ...neutralized. The B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization...cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...happening.” In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses... -
CRISPR Plasmids - Drosophila
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...