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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 955 results for: tes

Showing: 81 - 100 of 955 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...transgene under CMV Bamburg 16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb...

  2. Antibody Plasmid Collection

    Type
    Collection
    ...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...

  4. Distribution to Industry

    Type
    Collection
    ...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

  6. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

  7. Open Enzyme Collection

    Type
    Collection
    ...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

  8. Ras Pathway

    Type
    Collection
    ...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

  9. Lentivirus Plasmids

    Type
    Collection
    ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...

  10. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find... Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions, but what about...

  11. Kit Free RNA Extraction

    Type
    Protocol
    ...Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes,...residual ethanol wash and add RNase-free water or TE as soon as the entire tube is dried but while the...visible. Resuspend RNA pellet in RNase-free water or TE. Quantify and assess the quality of your RNA sample...Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000 × g at 4°C ...Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000 × g at 4°C to separate...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Reddy TE, Gersbach CA (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes... M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA (2017) CRISPR–Cas9 epigenome editing...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes...-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted...transcription. Created with BioRender.com. Tet1 initiates cytosine demethylation of DNA. However, several...ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Thakore PI, Perez-Pinera P, Brown MT, Majoros WH, Reddy TE, Gersbach CA. Correction of dystrophin expression...Ousterout DG, Kabadi AM, Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome ...grip strength, specific force, and other muscle tests, muscle function is clearly improved, although not...at the ten highest-ranking predicted off-target sites. Each paper characterizes additional, unique facets...

  16. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...from 40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...The SPARC system uses competitive recombination sites to selectively remove a stop cassette upstream of...expressed. By truncating one of the recombination sites in the construct to different lengths, the density... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...

  17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, ...neutralized. The B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization...cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...happening.”  In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses...

  18. Pouring LB Agar Plates

    Type
    Protocol
    ...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    ...your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent ...

  20. DNA Quantification

    Type
    Protocol
    ...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...ranges from 1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop...
Showing: 81 - 100 of 955 results