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We narrowed to 481 results for: vent

Showing: 81 - 100 of 481 results

  1. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...F1000Research, 2014; Dolan, F100Research, 2016). These events have also started progress towards solutions. The... necessary to determine the best solutions. The event also culminated in a joint American Society for ...science-related fields. In particular, two recent events have the potential to influence both graduate students...many institutions to set postdoc wages. The second event of note is a ruling in August by the National Labor...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...genome and a cell that has had multiple integration events. In addition, promoter choice can also impact transgene... approach can discriminate multiple integration events, reducing the possibility of underestimating the... are used to quantify the number of integration events. Another drawback to this method can be the absence...of infection (MOI) for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... with enhanced sensitivity for unitary synaptic events By Brian O’Neill The Lin Lab and collaborators ...microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials...While studying mEPSPs has been possible with conventional electrophysiological methods, it has remained... with enhanced sensitivity for unitary synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...designed small RNA, or guide RNA (gRNA). The gRNA conventionally contains a 20-nucleotide sequence that is complementary...becomes complexed with which variant of Cas9. To circumvent this problem, previous studies have proposed ...orthologs are less well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation of the CST...specimen, A= anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy...less photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition...developed the 3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics   ...produces beta-lactamase to degrade amp and therefore prevent cell death. One of the cool things about amp is...susceptible to degradation in agar plates. This prevents satellite colonies from forming while effectively...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...discipline. Getting companies to come to these types of events can be very tough. First I needed to find the right...least read my emails. Then pitching myself and the event was very difficult and somewhat awkward in the beginning...better with repetition. I learned to look at the event from the company’s perspective, focus on the message...professional updates. Whenever I see interesting events, articles and newsletters I make sure to share ...

  8. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos, mesothelioma...treatment As with many forms of cancer, the primary conventional treatments for mesothelioma include surgery ...This drug (known as an angiogenesis inhibitor) prevents the growth of new blood vessels required to sustain...treatment get, there still remains the problem of prevention. Asbestos exists pretty much everywhere, still...

  9. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...gRNA target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on ...the 3’ splice acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...and recombination events). TIDER can screen for specific homology-directed repair events and small edits... Fig. 2: Size screening for large deletion events.   Validating knock-in mutations You’ve introduced...combo and are hoping for homology-directed repair events, or maybe you’re performing a single base pair ...

  11. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...identifies a staggering ~250,000 phosphorylation events in the proteomes of higher mammals (2). How can... the importance of any of these phosphorylation events on the activity of a given protein? Classical methods...cysteine in their proteomes (3). This led to the eventual discovery of a naturally occurring phosphoserine-tRNA...inactive without requisite dual phosphorylation events on its activation loop, but can be made constitutively...

  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... the immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency syndrome (...an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and ...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... (Bedbrook & Ausubel, 1976). This recombination event produces a multimeric form of the plasmid with repeating...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...bind to the operator. The bound repressor then prevents transcription from occurring, which means that...to the tetracycline operator sequences (TetO), preventing transcription. In the presence of tetracycline...binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...

  15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ..., and publishing. The livestream video from the event can be found below along with a description of the...issues and their potential solutions. Prior to this event, I gave my own talk on reproducibility at Addgene... this booklet (which was also handed out at the event). Program 0 - 1:24 - Intro 1:24 - 29:31 - Reproducibility...recognizing that we’re biased and taking steps to prevent our biases and goals from effecting our experiments...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Nano-Lanterns including monitoring multiple cellular events such as the dynamics of subcellular structures ...NanoLuc® luciferase Many intracellular sensors of events like calcium release have been made using fluorescent...employing optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond ...intracellular calcium and is useful for direct imaging of events in cells and brain explants. This construct is ...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...provided by the chosen cell line to ensure safety and prevent the potential formation of replication-competent...insertional mutagenesis. However, random integration events can occur at a very low frequency. As with most... Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood...uses the cell machinery to produce new viruses, eventually lysing the cell to release them. Optogenetics...host cells as a result of a spontaneous crossover event between the rAAV vector and the adenoviral genes...

  18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was...between two lox2272 sites in the same orientation preventing the transgene from reverting. Figure created ...problem of DIO/FLEx vectors, the cause of these events, until recently, was not known. A recent paper ...2020) it was determined that the optimal titer to prevent off-target expression of the sensitive FLEx-split...

  19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...modification with an ssDNA substrate.  Gam: Gam prevents both the endogenous RecBCD and SbcCD nucleases...repressor gene, CI857, is used.  This mutant repressor prevents expression of the recombination genes at low temperatures...modify chromosomal genes. After the initial editing event, the defective prophage can be removed from the ...host E. coli by a second lambda red recombination event. Alternatively, if the modified allele is selectable...

  20. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...-stimulate mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics...antisense orientation with respect to the promoter to prevent its expression, and is flanked (“floxed”) by two...they targeted the dopamine (DA) neurons of the ventral tegmental area (VTA) of the brain. In particular...Optogenetic activation of dopamine neurons in the ventral tegmental area induces reanimation from general...
Showing: 81 - 100 of 481 results