We narrowed to 990 results for: Abo

...our adeno-associated viral vector guide to learn about AAV vector components, production and common uses...E2a and VA for replication. For more information about adenovirus, read our Adenoviral Vector Guide . Recombinant... factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog post on ...in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics 101 video...

...biology, laboratory research, and career development. eBooks Download any of our eBooks to learn about popular...Playlists include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes...

...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...building synthetic genetic circuits, engineering metabolic pathways, assembling gene-editing tools, and more...editing, functional genomics, and more complex metabolic engineering and synthetic biology, compatible ... Techniques by Nicola Patron at The Sainsbury Laboratory Weber E, Engler C, Gruetzner R, Werner S, Marillonnet...

...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

...Learn about bacterial and eukaryotic promoters, including transcription and the transcription complex...your gene of interest is expressed. Promoters are about 100–1,000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...E2a and VA for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral...linearized construct, and rAdV vectors can be harvested about 7–10 days later. Vogelstein designed multiple shuttle...

...the following guides to help you start learning about various scientific concepts related to our plasmid...Viral Vectors Find educational guides to learn more about the different types of viruses that are commonly...

... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

... design your own primers . Still have questions about choosing the best primer for your plasmid? Email...

...Learn about different molecular cloning techniques, focusing on seven common cloning methods....