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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 987 results for: des

Showing: 981 - 987 of 987 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Science Guides Viral Vectors AAV Vector Guide Adeno-Associated...inverted terminal repeats (ITRs). The Rep region encodes four non-structural replication proteins (Rep78...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins...cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...new window) Kwon, I., & Schaffer, D. V. (2008). Designer gene delivery vectors: molecular engineering and...., Schaffer, D. V., & Karpova, A. Y. (2016). A designer AAV variant permits efficient retrograde access...

  2. Cloning

    Type
    Guide
    ...for it to then be digested and ligated into your desired plasmid. It is important to note that restriction..., that cut your insert or backbone at only the desired location for your cloning project. Restriction ...rapidly shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase...clonase enzymes. Thousands of Gateway® destination plasmids have been made with different promoters, tags...polymerases synthesize DNA molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together...stranded DNA fragments can be used, so if properly designed, any insert fragment (PCR product or synthesized... backbone independent of how it was originally designed to be cloned into. A drawback of this system is...

  3. Optogenetics Guide

    Type
    Guide
    ... Science Guides Optogenetics Guide Optogenetics Guide Jump To: ...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific... Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins:....1 540 Plan Your Optogenetics Experiment When designing your optogenetics experiment, you’ll need to pick...key in optogenetic experiments. Your experimental design will determine whether you’ll need short or long... protein. This principle has also permitted the design of synthetic two-part transcription factors - Cry2...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...

  4. Adenovirus Guide

    Type
    Guide
    ... Science Guides Viral Vectors Adenoviral Vector Guide Adenoviral...most widely used viral vector platform for vaccine design against a diversity of viruses. This guide contains...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...be harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes...but has, however, proven to be very useful for designing and producing rAdV-based vaccines. Adenoviral ...altering gene activity. Oncolytic Referring to the destruction of cancer cells, generally by lysis. pAdEasy-...

  5. Promoters

    Type
    Guide
    ...gene. The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled...effects of expressing multiple genes at once. A well-designed screen can help you begin to understand what genes...important to a certain phenotype, and allow you to design additional hypothesis-directed experiments. As ...tube. Pooled libraries can be small, if they are designed to cover only a subset of genes, or very large...

  7. Educational Resources

    Type
    Guide
    ...educational resources, including eBooks, science guides, videos, and protocols...Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth instructional content on subjects...
Showing: 981 - 987 of 987 results