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We narrowed to 500 results for: RAP

Showing: 101 - 120 of 500 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses... read our secondary nanobodies blog post.  A rapid selection platform for generating sybodies against...developed a robust in-vitro protocol that enables rapid selection of synthetic nanobodies, called sybodies...for further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab...lab demonstrated they could use the protocol to rapidly identify and isolate sybodies targeting the SARS-CoV...BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, ...single domain antibodies for the conformational trapping of membrane proteins. eLife 7: . https://doi.org...

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https://doi.org...dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434... Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169...Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18(September...vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016/j.ymthe...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently developed...promoting domain. Each half was fused with one of the LOVTRAP domains. When both halves of the modified EB1 protein...system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9 cleavage...popularity of single base-editors has resulted in the rapid expansion of the genome editing toolkit. However...technologies across several cell types is the lack of a rapid and quantitative editing assay. To help accelerate...has deposited a reporter plasmid that allows for rapid fluorescence-based quantification of base editors...

  4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...of the VGS process and use cases in Human Gene Therapy. Existing methods for AAV quality control Historically...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038..., Paulk NK (2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded...Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb...Moullier P, Ayuso E, Vandenberghe LH (2017) AAV-ID: A Rapid and Robust Assay for Batch-to-Batch Consistency ...Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001...

  5. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... antibodies. This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV...anti-AAV antibodies often neutralize the therapeutic efficacy of AAV vectors. How are exosome AAVs produced...interest special contexts, such as the delivery of therapeutic proteins to the brain which requires crossing...cells using exosome-associated AAV." Molecular Therapy 25.2 (2017): 379-391. PubMed PMID: 28082074. PubMed...vector as a novel gene delivery system." Molecular Therapy 20.5 (2012): 960-971. PubMed PMID: 22314290. PubMed...Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    ...retro-orbital) to intermediate (tail vein) Intraperitoneal Intraperitoneal (IP) injections are given in the peritoneal...drugs and viral vectors. Like IV injections, intraperitoneal injections require a little bit of practice...purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds very ... that require ingestion, such as experimental therapies or pain relief, straight to the stomach. This ...retro-orbital) to intermediate (tail vein) Intraperitoneal Scruffing Peritoneal cavity 25–27 < 2–3 mL ...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...used for many years for in vivo delivery and gene therapy and represent one of the most studied viruses. ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...deficiency (most widely used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In...Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM,... Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry... of Cas9-Specific Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org/10.1089/hum.2015.087...

  9. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...over two decades, attempts to answer it in five paragraphs, with examples. If that sounds ambitious, that...Science” described in Chapter 3, and of course the rapidly changing world of genome engineering via CRISPR...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific... also spends some time on Addgene’s role in the rapid dissemination of these technologies, proposing that...are reading this review--in the current period of rapid flux, the chapter might still feel slightly dated...them wanting to learn more. There are individual paragraphs that may have warranted their own chapters, and...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...test for mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts with iPSCs...this approach is still not considered safe for therapeutic applications. piggyBac (PB) transposon: The PB...safer way to express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus...is considered a safer approach for developing therapeutic applications, but it can take a long time to ...induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295-305. PubMed ... cell generation. Expert opinion on biological therapy, 10 2, 231-42. PubMed PMID: 20088717. PubMed Central...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...CRISPR/Cas based COVID-19 testing methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using...incorporated into a lateral-flow readout slip that allows rapid detection.  Highlights: Cas13a is not catalytically...All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive and visual detection of novel coronavirus...operated, made accessible by the DIY-Bio movement. Rapid, field-deployable nucleobase detection and identification... D., Sharma, N., Kumar, M., … Maiti, S. (2020). Rapid, field-deployable nucleobase detection and identification..., & Alejandra, G. C. (2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...diagnostic tools and therapeutics. In addition, the strategic use of whole phages as therapeutics, which is also...long-term library storage. After overnight growth, scrape up, pool and extract plasmid DNA directly from ...Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in...drug approval pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed ...PMCID: PMC4728577. 4. Schmitz, Jonathan E., et al. "Rapid DNA library construction for functional genomic ...

  13. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ... in therapy. The lungs are difficult, but other tissues like the retina have cell-based therapies underway...researcher. Darrell's ultimate goal - to develop therapies to treat disease in the lung. Chapter 2: Balancing...disease through tissue repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary...Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the Kotton...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...genome engineering - such as certain forms of gene therapy - avoiding the use of viruses is also important...of each construct. This new tool kit allows the rapid generation of stable transgenic cell lines which... CD19-directed chimeric antigen receptor (CAR) therapy. SB is a valuable tool and an alternative to CRISPRs..., et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Biotechnol...PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade...

  15. How Addgene Creates Content

    Type
    Blog Post
    ...of text. Since our guides are heavily used, our graphic designer made most of the Antibody Guide figures...communication between our content team and our graphic designer to make sure things are clear, accurate...Fig. 2: Reference image (left) provided to our graphic designer upon request that the science communicator...design (right). Immediate and immense regret of the graphic designer not shown.   However, for our blog...retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. Thus, we are constantly...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is smaller than...simplicity of Cas12a makes it easier to package for therapeutic delivery by AAV, which has strict capacity limitations...sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement...TTTN. Best for: homology-directed repair or therapeutic delivery.   Cas14 – Mini, but single strand ...stirred up interest for its potential as a viral therapeutic that could specifically degrade target viral ...

  17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...analysis of the US Women’s Health Initiative Hormone Therapy Trial, for example, found that the study saved ...Reduced use of estrogen plus progestin hormone therapy resulted in 76,000 fewer cases of cardiovascular... cells and tissues, specifically in stem cell therapies. Let’s go back to why ten drugs were withdrawn... shows that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers... both sexes ensures that the diagnostics and therapeutic interventions you develop represent the whole...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...your study is aimed at developing human therapies. If a therapeutic is the end goal, it is best to work with...Targeting is also important in the development of therapies when expression must be restricted to specific...Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have drastically increased... A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical...

  19. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016... vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https://doi.org... liver, interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... that CRISPR technology is consistently showing rapid development and quick adoption by the community....request and use new tools. Because there’s such rapid adoption, we thought that requests would be biased...CRISPR tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, ...usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR community?  Addgene...looked at depositors and requestors by country, and graphed the top ten countries for each group (Figure 4)...
Showing: 101 - 120 of 500 results