...replicate plasmids of all types, but beyond that, researchers also use bacteria like E. coli and their relatively...to help you harness bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR...Purpose pRU Series Various Plasmids Cloned by researcher Fluorescent protein, GUS, and LacZ Derivatives... One-Hybrid Series Various Plasmids Cloned by researcher Growth of strain USO hisB- pyrF- rpoZ- on plates...Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase Keith Joung Transcription ...

...Find prime editor plasmids for (search and replace) genome editing....Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene editing method in which a reverse...tool from Hyongbum Kim's lab . Browse, sort, or search the tables below for CRISPR prime editing plasmids...editing are highlighted in the table below. Use the search bar to find a gRNA vector based on expression system...

...alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology...Epigenetics To make targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic... tables on Addgene's Empty gRNA Vectors page to search based on factors such as selectable marker or cloning...mammalian vectors, simply type "Mammalian" into the search bar. When using CRISPR, you will need to express...

... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

...recombinant antibodies to help facilitate scientific research. We are building a collection of affinity reagents...Addgene values transparency and accessibility in research and therefore makes the sequences of these affinity...of antibodies developed and validated for many research applications. Browse Antibodies Production Interested...

...Addgene values transparency and accessibility in research. For each antibody, we provide important details...their use, visit our Antibody Guide . Browse or search the table below or visit the following pages to...Antibodies targeting proteins for neuroscience research. Institute for Protein Innovation Collection :...

...nonprofit research organization with a mission to advance protein science to accelerate research and improve...detect, or localize particular proteins. Browse or search the table below to find antibodies targeting V5...

...adenovirus stocks. One major advantage of using AAV for research is that it is replication-limited and typically...plasmids for packaging your AAV product. You can search or this table based on plasmid name, capsid, and...required to produce infectious AAV particles. You can search or sort this table based on plasmid name, species...

...resources relevant to these organisms, or use our Search page to look for specific genes or applications...Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis...External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens...

...of posttranslational modifications in cells and research into its many roles in protein function and signaling...Söll labs at Yale University changed the way researchers can explore important questions surrounding serine...for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...

...constant regions of other antibodies. This has useful research applications, and is covered in the following ...conjugating reactions are possible. It is important to research your reaction and buffer before conjugating your...powerful, but in order to utilize it in the lab, researchers must be able to detect if and where an antibody...antibodies validated for a similar assay type) and search again. After filtering, narrow down further by ...

... the study of gene function, scientists were in search of inducible promoters capable of controlling eukaryotic...rapid induction with efficient tetracycline uptake. Search our collection for all tetracycline plasmids. Tet... models: improved animal models for biomedical research. Bockamp E, Maringer M, Spangenberg C, Fees S,...

...pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP Cre AND Flp Yes 137149 pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP Cre AND NOT...NOT Flp FRT/F5 Yes 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP Flp AND NOT Cre Yes 137151 pAAV-nEF-NpHR3.3...

...Retrograde construct from Alla Karpova at Janelia Research Institute....Cre-dependent Optogenetics Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CamKII Inhibitor Optogenetics Boyden...plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration with...

...transcription actually begins. Only eukaryotes and archaea, however, contain this TATA box. Most prokaryotes... proteins. Common Eukaryotic Promoters Used in Research Promoter Expression Description CMV Constitutive...controlled operon. Common Bacterial Promoters used in Research Promoter Expression Description T7 Constitutive...newest breakthroughs in plasmid technologies and research to overviews of molecular biology basics and plasmid...

...Neuroscience Neuroscience Antibody Collection Browse or search the table below to find antibodies currently available...catalog that target proteins popular in neuroscience research. These ready-to-use recombinant monoclonal antibodies...

...translational investment. All resources and high-quality research tools are shared openly. Plasmids from Emory-Sage-SGC... plasmids that are currently available. You can search or sort this table based on plasmid name, gene/...

...Consortium was established to promote continued research and development of engineered zinc finger technology...distribution to academic and nonprofit laboratories for research and educational use. Links to additional pages...

...turn impacts phenotype. The National Human Genome Research Institute (NHGRI) is funding this collaborative...window) is funded by the National Human Genome Research Institute (NHGRI) (Link opens in a new window)...

... Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by the table headings...Type PI Publication Back to Top Shuttle Vectors Search the table by keyword or sort by the table headings...